Download PDF
CC BY-NC-ND 4.0 · Rev Bras Ortop (Sao Paulo) 2023; 58(04): e653-e658
DOI: 10.1055/s-0042-1758359
Artigo Original
Trauma

Fracture in Irradiated Rat Femur: A Description of an Experimental Model and Evaluation of its Effectiveness

Article in several languages: português | English
Cláudio Beling Gonçalves Soares 
1   Médico Preceptor, Departamento de Cirurgia, Faculdade de Medicina Federal, Universidade de Minas Gerais, Belo Horizonte, MG, Brasil
,
Ivana Duval de Araújo 
2   Professor, Departamento de Cirurgia, Faculdade de Medicina, Universidade Federal, Minas Gerais, Belo Horizonte, MG, Brasil
,
Luiz Eduardo Moreira Teixeira 
2   Professor, Departamento de Cirurgia, Faculdade de Medicina, Universidade Federal, Minas Gerais, Belo Horizonte, MG, Brasil
,
Márcia Antoniazi Michelin 
3   Professor, Universidade Federal do Triângulo Mineiro, Instituto de Oncologia, Uberaba, MG, Brasil
,
Carla Jorge Machado 
2   Professor, Departamento de Cirurgia, Faculdade de Medicina, Universidade Federal, Minas Gerais, Belo Horizonte, MG, Brasil
,
Marcelo Peixoto Silveira 
4   Médico Ortopedista, Departamento de Ortopedia, Faculdade de Medicina, Federal, Universidade de Minas Gerais, Belo Horizonte, MG, Brasil
› Author Affiliations
› Further Information
Also available at
   Permissions and Reprints

Abstract

Objective We aim to describe an experimental model for studying femoral fractures in rats after exposure to ionizing radiation, demonstrating a way to apply a substance for analysis, the method for patterning fracture and irradiation, and how to evaluate its effectiveness based on radiographic studies.

Methods We used 24 rats divided into 2 groups of 12 animals each. The STUDY group was exposed to ionizing radiation and treated with saline solution, and the CONTROL group was not exposed to radiation and was treated with saline solution. All animals were subjected to standardized fracture of the right femur that was fixed with intramedullary wire. The efficiency of the bone union was assessed by radiographic exam.

Results Fracture healing was more efficient in bones not exposed to ionizing radiation (p = 0.012). All fractures met the criteria of being simple, diaphyseal, transverse or short oblique.

Conclusion The experimental model presented is an efficient alternative for the study of fractures in irradiated bones in rats.

Keywords

fracture healing - radiation effects - radiotherapy - fractures, spontaneous

Financial Support

The present research was partially funded by the Oncology Network of the Research Support Foundation of Minas Gerais (REONCO-FAPEMIG).




Publication History

Received: 02 June 2022

Accepted: 12 September 2022

Article published online:
04 July 2023

© 2023. Sociedade Brasileira de Ortopedia e Traumatologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

Thieme Revinter Publicações Ltda.
Rua do Matoso 170, Rio de Janeiro, RJ, CEP 20270-135, Brazil

 

  • Referências

  • 1 Soares C, Araújo I, Pádua B, Vilela C, Souza R, Teixeira L. Fratura patológica após radioterapia: Revisão sistemática da literatura. J Brazilian Med Assoc 2019; 65 (06) 902-908
    Search in Google Scholar
  • 2 Nicholls F, Ng AH, Hu S. et al. Can OP-1 stimulate union in a rat model of pathological fracture post treatment for soft tissue sarcoma?. J Orthop Res 2014; 32 (10) 1252-1263
    CrossrefPubMedSearch in Google Scholar
  • 3 Nicholls F, Janic K, Filomeno P, Willett T, Grynpas M, Ferguson P. Effects of radiation and surgery on healing of femoral fractures in a rat model. J Orthop Res 2013; 31 (08) 1323-1331
    CrossrefPubMedSearch in Google Scholar
  • 4 Lane JM, Sandhu HS. Current approaches to experimental bone grafting. Orthop Clin North Am 1987; 18 (02) 213-225
    CrossrefPubMedSearch in Google Scholar
  • 5 Dokmeci M, Kalender A, Sevimli R, Korkmaz M, Bilal O. The effect of ibandronate on fracture healing in rat tibia model. SM J Orthop 2016; 2 (04) 1041
    Search in Google Scholar
  • 6 Damy SB, Camargo RS, Chammas R, Figueiredo LF. Aspectos fundamentais da experimentação animal - aplicações em cirurgia experimental. Rev Assoc Med Bras 2010; 56 (01) 103-111
    CrossrefPubMedSearch in Google Scholar
  • 7 Blaes AH, Lindgren B, Mulrooney DA, Willson L, Cho LC. Pathologic femur fractures after limb-sparing treatment of soft-tissue sarcomas. J Cancer Surviv 2010; 4 (04) 399-404
    CrossrefPubMedSearch in Google Scholar
  • 8 Gortzak Y, Lockwood GA, Mahendra A. et al. Prediction of pathologic fracture risk of the femur after combined modality treatment of soft tissue sarcoma of the thigh. Cancer 2010; 116 (06) 1553-1559
    CrossrefPubMedSearch in Google Scholar
  • 9 Kerimoğlu S, Livaoğlu M, Sönmez B. et al. Effects of human amniotic fluid on fracture healing in rat tibia. J Surg Res 2009; 152 (02) 281-287
    CrossrefPubMedSearch in Google Scholar
  • 10 Karaçal N, Koşucu P, Cobanglu U, Kutlu N. Effect of human amniotic fluid on bone healing. J Surg Res 2005; 129 (02) 283-287
    CrossrefPubMedSearch in Google Scholar
  • 11 Aydin H, Saraçoğlu M, Kerimoğlu G, Kerimoğlu S, Topbaş M. [Effects of human amniotic fluid on posterolateral spinal fusion: an experimental preliminary study]. Eklem Hastalik Cerrahisi 2011; 22 (03) 166-171
    PubMedSearch in Google Scholar
  • 12 Bostrom MP, Saleh KJ, Einhorn TA. Osteoinductive growth factors in preclinical fracture and long bone defects models. Orthop Clin North Am 1999; 30 (04) 647-658
    CrossrefPubMedSearch in Google Scholar