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Horm Metab Res 2017; 49(05): 343-349
DOI: 10.1055/s-0043-102950
Endocrine Care
© Georg Thieme Verlag KG Stuttgart · New York

ANGPTL8 (Betatrophin) is Expressed in Visceral Adipose Tissue and Relates to Human Hepatic Steatosis in Two Independent Clinical Collectives

Christian von Loeffelholz
1   Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany
2   Integrated Research and Treatment Center, Center for Sepsis Control and Care (CSCC), Jena University Hospital, Jena, Germany and Department of Anaesthesiology and Intensive Care, Jena University Hospital, Jena, Germany
3   Department of Endocrinology, Diabetes, and Nutrition, Charité – Universitätsmedizin, Berlin, Germany
,
Andreas F. H. Pfeiffer
1   Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany
3   Department of Endocrinology, Diabetes, and Nutrition, Charité – Universitätsmedizin, Berlin, Germany
7   German Center for Diabetes Research (DZD), Neuherberg, Germany
,
Johan F. Lock
4   Department of General-, Visceral-, Vascular- and Paediatric Surgery, University Hospital of Wuerzburg, Wuerzburg, Germany
,
Steffi Lieske
5   Section of Metabolic and Vascular Medicine, Medical Clinic III, University Hospital Carl Gustav Carus, Dresden, Germany
,
Stephanie Döcke
1   Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany
,
Veronica Murahovschi
1   Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany
,
Jennifer Kriebel
6   Research Unit of Molecular Epidemiology, Helmholtz Zentrum Muenchen, German Research Center for Environmental Health, Neuherberg, Germany
7   German Center for Diabetes Research (DZD), Neuherberg, Germany
8   Institute of Epidemiology II, Helmholtz Zentrum Muenchen, German Research Center for Environmental Health, Neuherberg, Germany
,
Tonia de las Heras Gala
9   Research Unit of Diabetes Epidemiology, Institute of Epidemiology II, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany
,
Harald Grallert
6   Research Unit of Molecular Epidemiology, Helmholtz Zentrum Muenchen, German Research Center for Environmental Health, Neuherberg, Germany
7   German Center for Diabetes Research (DZD), Neuherberg, Germany
8   Institute of Epidemiology II, Helmholtz Zentrum Muenchen, German Research Center for Environmental Health, Neuherberg, Germany
,
Natalia Rudovich
1   Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany
3   Department of Endocrinology, Diabetes, and Nutrition, Charité – Universitätsmedizin, Berlin, Germany
,
Martin Stockmann
10   Department of General, Visceral and Transplantation Surgery, Charité – Universitätsmedizin, Berlin, Germany
,
Joachim Spranger
3   Department of Endocrinology, Diabetes, and Nutrition, Charité – Universitätsmedizin, Berlin, Germany
,
Gerhard Jahreis
11   Institute of Nutrition, Friedrich Schiller University, Jena, Germany
,
Stefan R. Bornstein
5   Section of Metabolic and Vascular Medicine, Medical Clinic III, University Hospital Carl Gustav Carus, Dresden, Germany
7   German Center for Diabetes Research (DZD), Neuherberg, Germany
,
George Lau
12   Humanity and Health GI and Liver Centre, University of Hong Kong, Hong Kong SAR, China
,
Aimin Xu
13   Department of Pharmacology & Pharmacy, University of Hong Kong, Hong Kong SAR, China
,
Jeanette Schulz-Menger
14   Department of Cardiology and Nephrology, Working Group on Cardiovascular Magnetic Resonance Imaging, Experimental and Clinical Research Center, Max-Delbrück-Centrum and Charité-Medical University Berlin and HELIOS Klinikum Berlin-Buch, Berlin, Germany
,
Louisa Exner
15   Institute of Clinical Pharmacology, Hannover Medical School, Hannover, Germany
,
Sven Haufe
15   Institute of Clinical Pharmacology, Hannover Medical School, Hannover, Germany
,
Jens Jordan
15   Institute of Clinical Pharmacology, Hannover Medical School, Hannover, Germany
18   Institute for Aerospace Medicine, German Aerospace Center, Cologne, Germany
,
Stefan Engeli   *
15   Institute of Clinical Pharmacology, Hannover Medical School, Hannover, Germany
,
Andreas L. Birkenfeld   *
5   Section of Metabolic and Vascular Medicine, Medical Clinic III, University Hospital Carl Gustav Carus, Dresden, Germany
7   German Center for Diabetes Research (DZD), Neuherberg, Germany
16   Competence Center for Metabolic Vascular Medicine Prof. Hanefeld, GWT- TU Dresden, Dresden, Germany
17   Section of Diabetes and Nutritional Sciences, King’s College London, London, UK
› Author Affiliations
Further Information

Publication History

received 20 August 2016

accepted 24 January 2017

Publication Date:
28 March 2017 (online)

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Abstract

Angiopoietin-like protein 8 (ANGPTL8)/betatrophin expression in visceral adipose tissue and associations with circulating fatty acid profile have not yet been investigated.

Forty subjects were included in a cross-sectional study, 57 in a dietary weight reduction intervention. Circulating Angiopoietin-like protein 8/betatrophin was measured in all subjects. Liver and adipose tissue were sampled and plasma fatty acids and tissue Angiopoietin-like protein 8/betatrophin expression were evaluated in the cross-sectional study. In the intervention study oral glucose testing and liver magnetic resonance scanning at baseline and after 6 months were performed. Angiopoietin-like protein 8/betatrophin mRNA was increased in visceral compared to subcutaneous adipose tissue (p<0.001). Circulating ANGPTL8/betatrophin correlated with liver steatosis (r=0.42, p=0.047), triacylglycerols (r=0.34, p=0.046), saturated (r=0.43, p=0.022), monounsaturated (r=0.51, p=0.007), and polyunsaturated fatty acids (r=−0.53, p=0.004). In the intervention study, baseline Angiopoietin-like protein 8/betatrophin correlated with age (r=0.32, p=0.010) and triacylglycerols (r=0.30, p=0.02) and was increased with hepatic steatosis (p=0.033). Weight loss reduced liver fat by 45% and circulating Angiopoietin-like protein 8/betatrophin by 11% (288±17 vs. 258±17 pg/ml; p=0.015). Angiopoietin-like protein 8/betatrophin is related to liver steatosis, while visceral adipose tissue represents an additional site of expression in humans.

Key words

diabetes - fatty acids - insulin resistance - fatty liver - hepatokine - HDL - LDL - triacylglycerides

* Equal contribution


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