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Horm Metab Res 2017; 49(07): 550-557
DOI: 10.1055/s-0043-106437
Endocrine Research
© Georg Thieme Verlag KG Stuttgart · New York

MicroRNA-524-5p Functions as a Tumor Suppressor in a Human Pituitary Tumor-Derived Cell Line

Wang Zhen   *
1   Department of Histology and Embryology, Medical school of Sun Yat-sen University, Guangzhou, Guangdong, China
2   Key Laboratory of Functional Molecules from Marine Microorganisms, Department of Education of Guangdong Province, Sun Yat-sen University, Guangzhou, Guangdong, China
,
Du Qiu   *
1   Department of Histology and Embryology, Medical school of Sun Yat-sen University, Guangzhou, Guangdong, China
2   Key Laboratory of Functional Molecules from Marine Microorganisms, Department of Education of Guangdong Province, Sun Yat-sen University, Guangzhou, Guangdong, China
,
Chen Zhiyong
3   Department of Neurosurgery and Pituitary Tumour Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China
,
Wang Xin
1   Department of Histology and Embryology, Medical school of Sun Yat-sen University, Guangzhou, Guangdong, China
2   Key Laboratory of Functional Molecules from Marine Microorganisms, Department of Education of Guangdong Province, Sun Yat-sen University, Guangzhou, Guangdong, China
,
Jian Mengyao
1   Department of Histology and Embryology, Medical school of Sun Yat-sen University, Guangzhou, Guangdong, China
2   Key Laboratory of Functional Molecules from Marine Microorganisms, Department of Education of Guangdong Province, Sun Yat-sen University, Guangzhou, Guangdong, China
,
Zhu Dimin
3   Department of Neurosurgery and Pituitary Tumour Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China
,
Hu Chonghui
1   Department of Histology and Embryology, Medical school of Sun Yat-sen University, Guangzhou, Guangdong, China
2   Key Laboratory of Functional Molecules from Marine Microorganisms, Department of Education of Guangdong Province, Sun Yat-sen University, Guangzhou, Guangdong, China
,
Wang Haijun
3   Department of Neurosurgery and Pituitary Tumour Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China
,
Zhu Yonghong
1   Department of Histology and Embryology, Medical school of Sun Yat-sen University, Guangzhou, Guangdong, China
2   Key Laboratory of Functional Molecules from Marine Microorganisms, Department of Education of Guangdong Province, Sun Yat-sen University, Guangzhou, Guangdong, China
› Author Affiliations
Further Information

Publication History

received 31 October 2016

accepted 08 March 2017

Publication Date:
04 May 2017 (online)

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Abstract

Clinical nonfunctional pituitary adenomas (NFAs) account for about 40% of pituitary adenomas with almost no clinically relevant hormonal symptoms. Increasing evidence shows that many microRNAs are involved in the development and progression of pituitary adenomas. MicroRNA-524-5p (miR-524-5p) has been reported to cause characteristic alterations in various tumors. However, the functional importance of miR-524-5p in NFAs remains unknown. The aim of this study was to explore the effects of overexpressing miR-524-5p on the proliferation, migration, invasion, and tumorigenicity of pituitary-derived folliculostellate (PDFS) cells using lentiviral transfection. Interestingly, the results showed that overexpressing miR-524-5p downregulated pituitary tumor-transforming gene 1 (PTTG1) binding factor (PBF) expression at both mRNA and protein levels and significantly attenuated cell proliferation, clonogenicity, migration, and invasion in vitro. Moreover, enhancing miR-524-5p blocked tumor growth in a nude mouse xenograft model in vivo. These findings suggest that miR-524-5p appears to play a critical role in the regulation of biological properties of PDFS cells, and may represent a potential therapeutic target for NFAs.

Key words

microRNA - pituitary adenoma - PBF

* These authors contributed equally to this work


 

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