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Horm Metab Res 2017; 49(08): 604-611
DOI: 10.1055/s-0043-109561
Endocrine Care
© Georg Thieme Verlag KG Stuttgart · New York

Measuring VLDL1-Triglyceride and VLDL2-Triglyceride Kinetics in Men: Effects of Dietary Control on Day-to-Day Variability

Rakel Fuglsang Johansen
1   Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus C, Denmark
,
Esben Søndergaard
1   Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus C, Denmark
2   The Danish Diabetes Academy, Odense, Denmark
,
Lars Peter Sørensen
1   Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus C, Denmark
,
Anne Grethe Jurik
3   Department of Radiology, Aarhus University Hospital, Aarhus C, Denmark
,
Søren Nielsen
1   Department of Endocrinology and Internal Medicine, Aarhus University Hospital, Aarhus C, Denmark
› Author Affiliations
Further Information

Publication History

received 11 January 2017

accepted 10 April 2017

Publication Date:
30 May 2017 (online)

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Abstract

The aim of this study was to assess the impact of dietary control on VLDL1- and VLDL2-TG kinetics and associated metabolic parameters. Twelve overweight/obese men were randomized to a provided 3 day isocaloric diet with fixed macronutrient composition (diet group, n=6) or to their regular unrestricted diet (non-diet group, n=6). VLDL1- and VLDL2-TG turnovers were measured twice 2–4 weeks apart, using primed-constant infusion of ex vivo labeled [1-14C]VLDL1-TG and [9,10-3H]VLDL2-TG. Isocaloric diet intervention lowered the difference in the mean of both VLDL2-TG secretion and clearance rate (p<0.01), and the coefficient of variation (CV) of VLDL2-TG clearance rate (p<0.05). The difference in mean and CV of the other kinetic estimates (VLDL1-TG secretion, clearance and oxidation rate) were lowered, but not significantly. The CV’s of total triglyceride, VLDL1-TG, and VLDL2-TG concentrations were significantly lowered by diet intervention compared to regular diet; total triglyceride (31%–5%, p<0.01), VLDL1-TG (42%–9%, p<0.01), and VLDL2-TG (36%–10%, p<0.01). In conclusion, VLDL1- and VLDL2-TG kinetics show great day-to-day variability, which may be diminished by diet intervention. Therefore, standardized macronutrient intake prior to study days improves the probability of demonstrating significant outcomes of cross-sectional and intervention studies of VLDL1-TG and VLDL2-TG kinetics and metabolism.

Key words

diet intervention - fatty acid/oxidation - human - triglycerides - VLDL kinetics

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