Int J Sports Med 2018; 39(02): 148-153
DOI: 10.1055/s-0043-120762
Genetics & Molecular Biology
© Georg Thieme Verlag KG Stuttgart · New York

Effects of the ACTN3 R577X Genotype on the Muscular Strength and Range of Motion Before and After Eccentric Contractions of the Elbow Flexors

Naoki Kikuchi*
1  Department of Training Science, Nippon Sport Science University, Tokyo, Japan
Yosuke Tsuchiya*
2  Faculty of Modern life, Teikyo Heisei University, Tokyo, Japan
Koichi Nakazato
3  Exercise Physiology, Nippon Sport Science University, Tokyo, Japan
Naokata Ishii
4  Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan
Eisuke Ochi
5  Faculty of Bioscience and Applied Chemistry, Hosei University, Tokyo, Japan
› Author Affiliations
Further Information

Publication History

accepted 25 September 2017

Publication Date:
17 November 2017 (eFirst)


The purpose of present study was to examine the association between ACTN3 R577X genotype and functional characteristics of elbow flexors before and after isokinetic eccentric contractions (ECCs). Fifty-two men (age: 20.8±3.8 years, height: 172.5±5.9 cm, body mass: 64.7±6.5 kg, BMI: 21.7±1.7) who had not participated in any regular resistance training for at least 1 year prior to this study were recruited. ECCs consisted of five sets of six maximal voluntary isokinetic (30°/s) ECCs of the elbow flexors with a range of motion (ROM) from 90° flexion to 0° (full extension). Measurements of maximal voluntary isometric contraction (MVC) torque, ROM, and muscle soreness were taken before, immediately after, and 1, 2, 3, and 5 days after ECCs. Genotyping results were analyzed for identifying ACTN3 R577X polymorphism (rs1815739) using TaqMan approach. The genotype frequencies of the ACTN3 R577X polymorphism were RR 26.9% (n=14), RX 50.0% (n=26), and XX 23.1% (n=12). There were no significant differences in MVC torque, ROM, and soreness between three genotype groups of ACTN3 R577X. However, MVC at baseline was greater in RR homozygotes than in X-allele carriers (combined XX and RX; p<0.05). ROM in RR homozygotes at baseline was lower than that of X-allele carriers. Although a significant decrease in ROM was observed in X-allele carriers until 3 days after ECCs, a significant ROM reduction in RR homozygotes was observed only immediately after ECCs. Our data indicated that ACTN3 RR genotype has higher MVC and lower flexibility than X-allele carriers at baseline, but the effect of ACTN3 R577X genotype on these two parameters is limited after ECCs.

* Authors contributed equally