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CC BY 4.0 · Journal of Health and Allied Sciences NU 2024; 14(01): 071-077
DOI: 10.1055/s-0043-1762581
Original Article

Aerobic Exercise Modulates Visceral Adipose Tissue of Estrogen Deprived Rats in an Experimental Model of Dyslipidemia

Walkyria Villegas Magalhães
1   Laboratory of Morphological and Immunohistochemical Studies, Department of Physical Education, São Judas Tadeu University located at Rua Taquari, Mooca, São Paulo, Brazil
,
Kemily Loren Barros Chucata
1   Laboratory of Morphological and Immunohistochemical Studies, Department of Physical Education, São Judas Tadeu University located at Rua Taquari, Mooca, São Paulo, Brazil
,
Nuha Ahmad Dsouki
2   Department of Morphology and Physiology of the ABC District Medical School located at Av. Lauro Gomes, Vila Sacadura Cabral, Santo André, Brazil
,
Ricardo Aparecido Baptista Nucci 
1   Laboratory of Morphological and Immunohistochemical Studies, Department of Physical Education, São Judas Tadeu University located at Rua Taquari, Mooca, São Paulo, Brazil
3   Department of Pathology, University of São Paulo Medical School located at Av. Dr. Arnaldo, Cerqueira César, São Paulo, Brazil
,
Aparecida Gabriela Bexiga Veloso 
4   Department of Morphology of Oswaldo Cruz School located at Rua Brigadeiro Galvão, Barra Funda, São Paulo, Brazil
,
Fernando Luiz Affonso Fonseca
5   Laboratory of Clinical Analysis of the ABC District Medical School located at Av. Lauro Gomes, Vila Sacadura Cabral, Santo André, Brazil
6   Department of Pharmaceutical Sciences of the Federal University of São Paulo (UNIFESP) located at Rua Sena Madureira, Vila Clementino, São Paulo, Brazil
,
Laura Beatriz Mesiano Maifrino 
5   Laboratory of Clinical Analysis of the ABC District Medical School located at Av. Lauro Gomes, Vila Sacadura Cabral, Santo André, Brazil
7   Dante Pazzanese Institute of Cardiology located at Av. Dr. Dante Pazzanese, Vila Mariana, São Paulo, Brazil
› Author Affiliations Funding None.
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Abstract

Introduction Menopausal women have an increase deposition of body fat and changes in the lipid profile, being especially susceptible to cardiovascular diseases, and type 2 diabetes. However, physical activity can mitigate this situation. Thus, the aim of the present study is to evaluate the effects of moderate aerobic exercise on visceral adipose tissue (VAT) of female LDL-receptor knockout ovariectomized mice. Methods We used 48 animals, divided into six groups (n = 8/per group): sedentary control (SC), sedentary ovariectomized control (SCO), trained ovariectomized control (TCO), sedentary non-ovariectomized LDL-receptor knockout (KS), sedentary ovariectomized LDL-receptor knockout (KOS), and trained LDL-receptor knockout ovariectomized (KOT). We analyzed the VAT through morphometric and stereological parameters in hematoxylin and eosin stained sections. Additionally, we evaluated biochemical parameters as glucose, triglycerides, and total cholesterol. Finally, immunohistochemical techniques for matrix remodeling, inflammation, apoptosis, and oxidative stress were evaluated. Results We observed that menopause is related to increased visceral adiposity, inflammation, oxidative stress, macrophages activity, serum levels of glucose, triglycerides, and total cholesterol. However, exercise was effective in reducing these parameters, as well as being associated with increased vascularization of VAT and interstitial volume density. Conclusions Moderate exercise is a key factor in mitigating the effects of dyslipidemia in estrogen deprivation. However, further studies are needed to corroborate with our findings.

Keywords

adiposity - dyslipidemia - experimental - menopause - treadmill

Availability of Data and Materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.


Authors' contributions

W.V.M. Investigation, writing-review and editing.


K.L.B.C. Investigation, writing-review and editing.


N.A.D. Investigation, writing-review and editing.


R.A.B.N. Data curation, formal analysis, visualization, writing-review and editing.


A.G.B.V. Investigation, writing-review and editing.


F.L.A.F. Resources, supervision, validation, writing-original draft.


L.B.M.M. Conceptualization, methodology, project administration, writing-original draft.




Publication History

Article published online:
14 April 2023

© 2023. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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