Characterization of TRP channels in patients with chronic rhinosinusitis (CRS).

Transient receptor potential (TRP) channels are transmembrane ion channels activated by influx of calcium ions. There are 28 different channels known in mammals, divided into 6 subfamilies. They play an important role in various physiological processes in temperature, as well as pain perception. Furthermore, a direct influence on T-cell level is being discussed suggesting a possible role in the pathomechanism of chronic rhinosinusitis (CRS). However, the exact distribution of the different channels in the various subtypes of CRS is still unclear. The aim of the present study was to specifically characterize TRP channels. Quantification of TRPM4, TRPV1 and TRPA1 expression was performed in a total of 14 subjects diagnosed with chronic rhinosinusitis with nasal polyps (CRSwNP), chronic rhinosinusitis without nasal polyps (CRSsN) and nasal mucosa of healthy subjects as controls. For this purpose, tissue obtained intraoperatively during pansinus surgery was prepared and RNA was isolated using QuiagenRNeasy Kit (Qiagen GmbH, Germany). Subsequently, real-time qPCR was performed for quantification. TRPM4, TRPV1 and TRPA1 channels were detected in all samples. So far, we could not detect any significant differences between the subgroups, however, a higher concentration of the upper mentioned TRP subfamilies, especially TRPM4, is shown in the inflammatory altered tissue in comparison to healthy controls. TRP channels can be detected in all study groups in different concentrations, but without significant differences. Further studies are neede to determine all known TRP channels to establish a targeted map of the TRP channels in CRS patients and to get more information regarding the involvement of these channels in the pathophysiology of CRS.

Publication History

Article published online:
12 May 2023