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CC BY 4.0 · Journal of Health and Allied Sciences NU 2024; 14(02): 210-218
DOI: 10.1055/s-0043-1769511
Original Article

Nephroprotective Potential of Sphaeranthus indicus Linn Extract against Hyperglycemia and Dyslipidemia in Streptozotocin-Induced Diabetic Nephropathy

Autoren

  • Vishal B. Jadhav 

    1   Department of Pharmacology, Bhupal Nobles' College of Pharmacy, Bhupal Nobles' University, Udaipur, Rajasthan, India

  • Jai Singh Vaghela 

    1   Department of Pharmacology, Bhupal Nobles' College of Pharmacy, Bhupal Nobles' University, Udaipur, Rajasthan, India

Funding None.
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Abstract

Objective This study was aimed at determining the nephroprotective potential of Sphaeranthus indicus Linn methanol extract (SME) against hyperglycemia and dyslipidemia in streptozotocin (STZ)-induced diabetic nephropathy (DNP) in adult Wistar albino rats.

Materials and Methods Following STZ-induced diabetes, adult albino Wistar rats of either sex with serum glucose level more than 250 mg/dL were chosen and randomized into six groups (n = 6 rats per group) and received the treatment as follows: Group I: Normal nondiabetic (ND) rats received a single intraperitoneal dose of citrate buffer in the same volume as STZ and 1% (w/v) carboxymethyl cellulose (CMC) per os (po), group II: diabetic (STZ) control rats received oral dosage of 1% (w/v) CMC, group III, IV and V: STZ + SME treated rats received a suspension of SME (100, 200, and 400 mg/kg, po) in 1% (w/v) CMC, and group VI: STZ + MET treated rats received metformin (500 mg/kg, po) as suspension in 1% (w/v) CMC. From 28th day to the 56th day of STZ injection, SME and MET were given for 28 days in the form of freshly prepared suspension. The impact of STZ-induced DNP was analyzed through the estimation of body weight, serum glucose, and hemoglobin A1c levels, renal functional parameters, the serum lipid profile, oxidative stress markers, and analysis of renal histoarchitecture.

Result Diabetic (STZ) control rats showed significant alterations in body weight, serum glucose and hemoglobin A1c levels, renal functional parameters, the serum lipid profile, oxidative stress markers, and renal histoarchitecture in contrast to normal ND rats. SME and MET treatment significantly reduced hyperglycemia-induced enhanced lipid profile and oxidative stress, normalized renal functional parameters, and restored renal histoarchitecture by reducing vacuolar degeneration of renal tubules in contrast to diabetic (STZ) control rats. These findings were attributed to SME's efficacy in DNP.

Conclusion In STZ-sensitized diabetic rats, SME retarded the progress of nephropathy. The observed nephroprotective potential of SME is ascribed to its hypoglycemic, hypolipidemic, and antioxidant activities.

Keywords

streptozotocin - diabetic nephropathy - Sphaeranthus indicus - hypoglycemic - antioxidant - hypolipidemic

Ethics Approval

The protocol No. 24/BNCP/IAEC/2021 of the experiment was approved by the Institutional Animal Ethics Committee (IAEC) of B. N. College of Pharmacy, Bhupal Nobles' University, Udaipur-313001, Rajasthan (India) (870/PO/Re/S/05/CPCSEA).


Availability of Data and Materials

The datasets used and/or analyzed during this study are available from the corresponding author on reasonable request.


Authors' Contributions

All authors contributed to the study conception and design. Experimental studies were conducted by VBJ. Data collection and analysis were performed by VBJ and JSV. The first draft of the manuscript was written by VBJ under the supervision of JSV. Both the authors commented on previous versions of the manuscript. Both the authors read and approved the final manuscript.




Publikationsverlauf

Artikel online veröffentlicht:
13. Juni 2023

© 2023. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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