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DOI: 10.1055/s-0043-1771272
High-Fidelity Perforator Visualization for Cadaver Dissection in Surgical Training

Abstract
In the first half of the third century B.C., Herophilus and Erasistratus performed the first systematic dissection of the human body. For subsequent centuries, these cadaveric dissections were key to the advancement of anatomical knowledge and surgical techniques. To this day, despite various instructional methods, cadaver dissection remained the best way for surgical training. To improve the quality of education and research through cadaveric dissection, our institution has developed a unique method of perforator-preserving cadaver injection, allowing us to achieve high-fidelity perforator visualization for dissection studies, at low cost and high efficacy. Ten full body cadavers were sectioned through the base of neck, bilateral shoulder, and hip joints. The key was to dissect multiple perfusing arteries and draining veins for each section, to increase “capture” of vascular territories. The vessels were carefully flushed, insufflated, and then filled with latex dye. Our injection dye comprised of liquid latex, formalin, and acrylic paint in the ratio of 1:2:1. Different endpoints were used to assess adequacy of injection, such as reconstitution of eyeball volume, skin turgor, visible dye in subcutaneous veins, and seepage of dye through stab incisions in digital pulps. Dissections demonstrated the effectiveness of the dye, outlining even the small osseous perforators of the medial femoral condyle flap and subconjunctival plexuses. Our technique emphasized atraumatic preparation, recreation of luminal space through insufflation, and finally careful injection of latex dye with adequate curing. This has allowed high-fidelity perforator visualization for dissection studies.
Authors' Contributions
A.W.J.W.: surgeon, design of study, data collection and analysis, interpretation of data, drafting the manuscript, approval of manuscript.
Y.O.K.: surgeon, design of study, data collection, interpretation of data, drafting and revising the manuscript, approval of manuscript.
K.Y.C.: data collection, interpretation of data, drafting and revising the manuscript, approval of manuscript.
B.K.T.: interpretation of data, drafting and revising the manuscript, approval of manuscript.
Ethical Approval
This was not required for the study.
Patient Consent
Since bodies were deceased and unidentified, consent was not necessary and not applicable.
Publikationsverlauf
Eingereicht: 30. September 2022
Angenommen: 15. Juni 2023
Artikel online veröffentlicht:
31. August 2023
© 2023. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)
Thieme Medical Publishers, Inc.
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References
- 1 von Staden H. The discovery of the body: human dissection and its cultural contexts in ancient Greece. Yale J Biol Med 1992; 65 (03) 223-241
- 2 Taylor GI, Palmer JH. The vascular territories (angiosomes) of the body: experimental study and clinical applications. Br J Plast Surg 1987; 40 (02) 113-141
- 3 Saint-Cyr M, Wong C, Schaverien M, Mojallal A, Rohrich RJ. The perforasome theory: vascular anatomy and clinical implications. Plast Reconstr Surg 2009; 124 (05) 1529-1544
- 4 Estai M, Bunt S. Best teaching practices in anatomy education: a critical review. Ann Anat 2016; 208: 151-157
- 5 Singh M, Sharma M, Nair AG. et al. Cadaver dissection for oculoplastic procedures: a beginner's guide. Indian J Ophthalmol 2022; 70 (09) 3239-3244
- 6 Are C, Stoddard HA, Thompson JS, Todd GL. The influence of surgical demonstrations during an anatomy course on the perceptions of first-year medical students toward surgeons and a surgical career. J Surg Educ 2010; 67 (05) 320-324
- 7 Song YK, Jo DH. Current and potential use of fresh frozen cadaver in surgical training and anatomical education. Anat Sci Educ 2022; 15 (05) 957-969
- 8 American Association of Tissue Banks. AATB Standards for Tissue Banking. 14th edition. McLean, VA: American Assocation of Tissue Banks; 2016
- 9 Watanabe K, Tabira Y, Saga T. et al. Novel cadaver injection method using latex and magnetic fluid. Kurume Med J 2018; 64 (1.2): 39-43
- 10 Doomernik DE, Kruse RR, Reijnen MM, Kozicz TL, Kooloos JG. A comparative study of vascular injection fluids in fresh-frozen and embalmed human cadaver forearms. J Anat 2016; 229 (04) 582-590