Abstract
Objectives Inflammation of the dental pulp tissue caused by bacteria, creating an immunology
response of death of the dental pulp, is called apoptosis. The Porphyromonas gingivalis that cause apoptosis is lipopolysaccharide (LPS) through toll-like receptor (TLR)
via two different mechanisms, intracellular and extracellular pathways. This study
analyzed the role of LPS exposure of neuron cells, tumor necrosis factor-α (TNF-α),
and cytochrome c (cyt-c) expression in the dental pulp to predict the possible mechanism
of apoptosis.
Materials and Methods The lower tooth of Sprague Dawley rats was opened and exposed to LPS for 48 hours.
Then the neuron cell analyzed histopathology using hematoxylin–eosin, whereas the
TNF-α and cyt-c expression with indirect immunohistochemistry using a light microscope.
The relationship between neuron cells with TNF-α and cyt-c was analyzed using stepwise
regression linear analysis.
Result The LPS exposure showed a lower number of neuron cells and had a relationship with
TNF-α expression but not with cyt-c, while compared with control, both TNF-α and cyt-c
expression were higher in neuron cells.
Conclusion LPS exposure in dental pulp is possible to stimulate the apoptosis process through
extracellular pathways marked by higher TNF-α expression.
Keywords
apoptosis - TNF-α - cytochrome c - dental pulp - neuron cell - immunology
