Download PDF
CC BY 4.0 · Eur J Dent
DOI: 10.1055/s-0044-1779425
Original Article

Potency of Hyperbaric Oxygen Therapy on Oral Candidiasis Diabetes Mellitus

Kristanti Parisihni 
1   Department of Oral Biology, Faculty of Dentistry, Universitas Hang Tuah, Surabaya, Indonesia
,
Dian Mulawarmanti 
1   Department of Oral Biology, Faculty of Dentistry, Universitas Hang Tuah, Surabaya, Indonesia
,
Kharinna Widowati 
2   Department of Oral Medicine, Faculty of Dentistry, Universitas Hang Tuah, Surabaya, Indonesia
› Author Affiliations Funding This research was supported by Internal Research Program Grant, funded by Universitas Hang Tuah Academic Year 2022/2023.
› Further Information
   Permissions and Reprints

Abstract

Objectives This study aimed to determine the potency of hyperbaric oxygen therapy (HBOT) to the blood glucose level, amount of lymphocytes, and the expression of antibody anti-Candida in oral candidiasis diabetes mellitus.

Materials and Methods Thirty Wistar rats were divided into five groups: K1 normal-control group, while K2, P1, P2, and P3 were diabetes induced by streptozotocin 50 mg/kg body weight. Oral candidiasis was induced by inoculation 0.1 mL Candida albicans ATCC 10321 on dorsum of the tongue. P1 was given nystatin oral suspension, P2 was treated by HBOT 2.4 ATA, 3 × 30 minutes each with a 5-minute interval, and P3 was treated by the combination of nystatin and HBOT. All the treatments were performed for 5 consecutive days. Fasting blood glucose level was examined on glucometer strips, lymphocyte was examined from complete blood count, and the expression of antibody anti-Candida was examined on immunohistochemistry staining

Statistical Analysis Data analyzed by a one-way analysis of variance and least significant difference test with the result of p-value less than 0.05 was considered to be statistically significant.

Results HBOT reduced blood glucose level (p > 0.05) increased the amount of lymphocyte (p < 0.05). All treatments reduced the expression of antibody anti-Candida (p < 0.05) with the best result on combination of HBOT with nystatin.

Conclusion HBOT decreased the blood glucose level, increased the amount of lymphocytes, and reduced the expression of antibody anti-Candida in oral candidiasis diabetes mellitus.

Keywords

hyperbaric oxygen therapy - oral candidiasis - diabetes mellitus - lymphocytes - hyperglycemia


Publication History

Article published online:
31 March 2024

© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

Thieme Medical and Scientific Publishers Pvt. Ltd.
A-12, 2nd Floor, Sector 2, Noida-201301 UP, India

 

  • References

  • 1 GBD 2021 Diabetes Collaborators. Global, regional, and national burden of diabetes from 1990 to 2021, with projections of prevalence to 2050: a systematic analysis for the Global Burden of Disease Study 2021. Lancet 2023; 402 (10397): 203-234
    CrossrefPubMedGoogle Scholar
  • 2 Nazir MA, AlGhamdi L, AlKadi M, AlBeajan N, AlRashoudi L, AlHussan M. The burden of diabetes, its oral complications and their prevention and management. Open Access Maced J Med Sci 2018; 6 (08) 1545-1553
    CrossrefPubMedGoogle Scholar
  • 3 Rodrigues CF, Rodrigues ME, Henriques M. Candida sp. infections in patients with diabetes mellitus. J Clin Med 2019; 8 (01) 76
    CrossrefPubMedGoogle Scholar
  • 4 Dunne MR, Wagener J, Loeffler J, Doherty DG, Rogers TR. Unconventional T cells - new players in antifungal immunity. Clin Immunol 2021; 227: 108734
    CrossrefPubMedGoogle Scholar
  • 5 Aitken-Saavedra J, Lund RG, González J. et al. Diversity, frequency and antifungal resistance of Candida species in patients with type 2 diabetes mellitus. Acta Odontol Scand 2018; 76 (08) 580-586
    CrossrefPubMedGoogle Scholar
  • 6 Verhulst MJL, Loos BG, Gerdes VEA, Teeuw WJ. Evaluating all potential oral complications of diabetes mellitus. Front Endocrinol (Lausanne) 2019; 10 (02) 56
    CrossrefPubMedGoogle Scholar
  • 7 Baitule S, Patel AH, Murthy N. et al. A systematic review to assess the impact of hyperbaric oxygen therapy on glycaemia in people with diabetes mellitus. Medicina (Kaunas) 2021; 57 (10) 1134
    CrossrefPubMedGoogle Scholar
  • 8 Mulawarmanti D, Parisihni K, Widyastuti W. The impact of hyperbaric oxygen therapy on serum c-reactive protein levels, osteoprotegerin expression, and osteoclast numbers in induced-periodontitis diabetic rats. Eur J Dent 2020; 14 (03) 404-409
    Article in Thieme ConnectPubMedGoogle Scholar
  • 9 Pargaputri AF, Andriani D. Pengaruh pemberian terapi oksigen hiperbarik terhadap jumlah limfosit darah pada kandidiasis oral Imunosupresi model. Jurnal Kedokteran Gigi 2018; 12 (02) 117-125
    Google Scholar
  • 10 Maqbool M, Mir SA. ‘Animal models of diabetes mellitus: an overview’, Nippon rinsho. Jpn J Clin Med 2019; 9 (01) 472-475
    Google Scholar
  • 11 Srinivasan K, Ramarao P. Animal models in type 2 diabetes research: an overview. Indian J Med Res 2007; 125 (03) 451-472
    PubMedGoogle Scholar
  • 12 Kohli S, Shinde CV. Candidiasis and oral cavity: a review. Int J Res Rev 2019; 6 (05) 335-340
    Google Scholar
  • 13 Rai A, Misra SR, Panda S. et al. Nystatin effectiveness in oral candidiasis treatment: a systematic review & meta-analysis of clinical trials. Life (Basel) 2022; 12 (11) 1677
    Google Scholar
  • 14 Liu Y, Zhang D, Yuan J. et al. Hyperbaric oxygen ameliorates insulin sensitivity by increasing GLUT4 expression in skeletal muscle and stimulating ucp1 in brown adipose tissue in T2DM mice. Front Endocrinol (Lausanne) 2020; 11 (32) 32
    CrossrefPubMedGoogle Scholar
  • 15 Wei R, Li P, Xue Y, Liu Y, Gong W, Zhao W. Impact of diabetes mellitus on the immunity of tuberculosis patients: a retrospective, cross-sectional study. Risk Manag Healthc Policy 2022; 15 (01) 611-627
    CrossrefPubMedGoogle Scholar
  • 16 Koyasu S, Moro K. Role of innate lymphocytes in infection and inflammation. Front Immunol 2012; 3 (05) 101
    PubMedGoogle Scholar
  • 17 Khanam A, Hithamani G, Naveen J, Pradeep SR, Barman S, Srinivasan K. Management of invasive infections in diabetes mellitus: a comprehensive review. Biologics 2023; 3: 40-71
    CrossrefGoogle Scholar
  • 18 Ernawati DS, Surboyo MDC, Ayuningtyas NF, Nagoro AAB. Role of inflammatory cell responses in stimulating fibroblasts in diabetic oral ulcer after treatment with liquid smoke of coconut endocarp: a histological assessment. Eur J Dent 2021; 15 (01) 71-76
    Article in Thieme ConnectPubMedGoogle Scholar
  • 19 Ortega MA, Fraile-Martinez O, García-Montero C. et al. A General overview on the hyperbaric oxygen therapy: applications, mechanisms and translational opportunities. Medicina (Kaunas) 2021; 57 (09) 864
    CrossrefPubMedGoogle Scholar
  • 20 Woo J, Min JH, Lee YH, Roh HT. Effects of hyperbaric oxygen therapy on inflammation, oxidative/antioxidant balance, and muscle damage after acute exercise in normobaric, normoxic and hypobaric, hypoxic environments: a pilot study. Int J Environ Res Public Health 2020; 17 (20) 7377
    CrossrefPubMedGoogle Scholar
  • 21 Korpinar S, Uzun H. The effect of hyperbaric oxygen at different pressures on oxidative stress and antioxidant status in rats. Medicina (B Aires) 2019; 55 (02) 1-8
    Google Scholar
  • 22 Li FQ, Ma CF, Shi LN. et al. Diagnostic value of immunoglobulin G antibodies against Candida enolase and fructose-bisphosphate aldolase for candidemia. BMC Infect Dis 2013; 13: 253
    CrossrefPubMedGoogle Scholar
  • 23 Samaranayake YH, Samaranayake LP. Experimental oral candidiasis in animal models. Clin Microbiol Rev 2001; 14 (02) 398-429
    CrossrefPubMedGoogle Scholar
  • 24 Laihad FM, Sudiana IK, Guritno MS. Literature review: hyperbaric oxygen therapy on mucormycosis infection in oral cavity. Folia Medica Indonesiana. 2017; 53 (02) 163-168
    CrossrefGoogle Scholar
  • 25 Andriani D, Setyaningtyas D. Nafi’ah. Efek ekstrak acanthus ilicifolius terhadap ekspresi antibodi anticandida albicans pada tikus wistar terimunosupresi oral kandidiasis. Denta Jurnal Kedokteran Gigi 2017; 11 (01) 35-41
    Google Scholar
  • 26 Segal E, Menhusen MJ, Shawn S. Hyperbaric oxygen in the treatment of invasive fungal infections: a single-center experience. Isr Med Assoc J 2007; 9 (05) 355-357
    PubMedGoogle Scholar
  • 27 Forsyth CB, Mathews HL. Lymphocyte adhesion to Candida albicans . Infect Immun 2002; 70 (02) 517-527
    CrossrefPubMedGoogle Scholar
  • 28 Dhingra S, Buckey JC, Cramer RA. Hyperbaric oxygen reduces Aspergillus fumigatus proliferation in vitro and influences in vivo disease outcomes. Antimicrob Agents Chemother 2018; 62 (03) 17-18
    CrossrefPubMedGoogle Scholar