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CC BY 4.0 · Eur J Dent
DOI: 10.1055/s-0044-1801280
Original Research

Osteogenesis Potential of Polymethylmethacrylate–Hydroxyapatite and Stem Cells from Human Exfoliated Deciduous Teeth as Alveolar Bone Graft: An In Silico Study

Tania Saskianti 
1   Department of Pediatric Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
2   Biomaterial and Tissue Engineering Research Center, Universitas Airlangga, Surabaya, Indonesia
,
Michelle Angelina
1   Department of Pediatric Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Ardianti Maartina Dewi 
1   Department of Pediatric Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Yulanda Antonius
3   Faculty of Biotechnology, University of Surabaya, Surabaya, Indonesia
,
Amelinda Nabila Zahri
1   Department of Pediatric Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Rini Devijanti Ridwan
2   Biomaterial and Tissue Engineering Research Center, Universitas Airlangga, Surabaya, Indonesia
4   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Masami Kanawa
5   Research and Development Division, Department of Biomedical Science, Natural Science Center for Basic Research and Development, Hiroshima University, Hiroshima, Japan
,
Takeshi Kawamoto
6   Writing Center, Hiroshima University, Hiroshima, Japan
,
Kotaro Tanimoto
7   Department of Orthodontics and Craniofacial Developmental Biology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan
,
Katsumi Fujimoto 
8   Department of Molecular Biology and Biochemistry, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan
› Author Affiliations Funding This research was supported by the International Research Network 2023 Research Grant from Universitas Airlangga (No: 1656/UN3.LPPM/PT.01.03/2023).
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Abstract

Objective The goal is to analyze the osteogenesis potential of polymethylmethacrylate (PMMA)–hydroxyapatite (HA) and stem cells from human exfoliated deciduous teeth (SHED) as a biomaterial candidate for alveolar bone defect therapy through a bioinformatic approach within an in silico study.

Materials and Methods Three-dimensional (3D) ligand structures consisting of HA, PMMA, and target proteins of SHED were obtained from the PubChem database. STITCH was used for SHED target protein analysis, STRING was utilized for analysis and visualization of protein pathways related to osteogenesis, PASS Online was employed to predict biological functions supporting osteogenesis potential, PyRx 0.8 was used for molecular docking analysis, and PyMol was utilized to visualize the 3D structures resulting from the molecular docking analysis.

Results PMMA ligand was found to support osteogenesis through several biological functions, while interaction of HA ligand with matrix metalloproteinase (MMP) 20, DSPP, IBSP, SPP1, CD44, and MMP7 protein was revealed to play a role specifically in extracellular matrix organization. The interaction of all these proteins played a role in various pathways of osteogenesis. Toxicity level predictions of PMMA and HA were at class V and class III, respectively, which means that both ligands were shown to be neither hepatotoxic, carcinogenic, immunotoxic, nor cytotoxic. However, the ligand of PMMA had a lower binding affinity to SHED's protein (MMP7, MMP20, CD44, BMP7, and COL1A1) than the control ligand.

Conclusion The interaction between HA–PMMA ligands and several SHED proteins showed biological process and osteogenesis pathways supporting the osteogenesis potential of PMMA–HA and SHED for alveolar bone defect therapy.

Keywords

in silico - human and health - polymethylmethacrylate - hydroxyapatite - stem cells from human exfoliated deciduous teeth - alveolar bone defect therapy


Publication History

Article published online:
12 March 2025

© 2025. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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