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CC BY 4.0 · Eur J Dent
DOI: 10.1055/s-0045-1802571
Original Research

Evaluation of the Cytotoxicity of a Newly Developed Obturating Material for Pulpectomy in Primary Teeth Using Embryonic Toxicology, Brine Shrimp Lethality, and MTT Assay: An In Vitro Study

Noor Fathima J
1   Department of Pedodontics and Preventive Dentistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India
,
Lavanya Govindaraju
1   Department of Pedodontics and Preventive Dentistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India
,
Ganesh Jeevanandan
1   Department of Pedodontics and Preventive Dentistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India
,
Prabhadevi C. Maganur
2   Division of Pediatric Dentistry, Department of Preventive Dental Sciences, College of Dentistry, Jazan University, Jazan, Saudi Arabia
,
Satish Vishwanathaiah
2   Division of Pediatric Dentistry, Department of Preventive Dental Sciences, College of Dentistry, Jazan University, Jazan, Saudi Arabia
,
Ali Ahmed Assiry
3   Department of Preventive Dental Science, Faculty of Dentistry, Najran University, Najran, Saudi Arabia
,
Ather Ahmed Syed
2   Division of Pediatric Dentistry, Department of Preventive Dental Sciences, College of Dentistry, Jazan University, Jazan, Saudi Arabia
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Abstract

Objective The search for an ideal obturating material has taken precedence due to the revolution of the materials used in pediatric endodontics. With zinc oxide, calcium hydroxide, and metronidazole as its core constituents, an unique obturating material was developed. To aid in the healing process, the material should be biocompatible and most importantly it should not have any negative consequences. Thus, using embryonic toxicology, brine shrimp lethality, and methylthiazol tetrazolium (MTT) assay, the current investigation sought to determine the cytotoxicity of the newly developed obturating material, which contained calcium hydroxide, zinc oxide, and metronidazole at 2% 60–40 concentrations.

Materials and Methods In zebrafish embryonic toxicology method and brine shrimp lethality assay, five distinct concentrations of the new obturating material was tested and compared with the control in a 24-well plate containing fertilized zebrafish eggs and in 6-well plate containing nauplii, respectively. The hatching and the viability rate of the zebrafish embryos and survival rate of nauplii were calculated. In MTT assay, the percentage of fibroblast cell viability and the cell morphology was documented. A statistical analysis was performed on all of the collected data.

Results The hatching and the viability rate of the zebrafish embryos falls as the concentration of the new obturating material rises. The survival rate of the nauplii also falls with rise in the concentration of the obturating material. No cytotoxic effect was demonstrated by the novel obturating material on the human gingival fibroblasts up to 200 μg/mL concentration.

Conclusion The novel obturating material exhibits minimal cytotoxic effects even at increased concentrations.

Keywords

cytotoxicity - MTT assay - obturation - primary teeth - pulpectomy


Publication History

Article published online:
12 March 2025

© 2025. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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  • References

  • 1 Lakshmanan L, Jeevanandan G, Maganur PC, Vishwanathaiah S. Fracture incidence of Kedo-S square pediatric rotary files: a prospective clinical study. Eur J Dent 2022; 16 (03) 594-598
    Thieme ConnectPubMedSearch in Google Scholar
  • 2 Mukundan D, Gurunathan D, Thangavelu L. Comparative evaluation of 1% sodium hypochlorite vs other intracanal irrigants during pulpectomy of primary teeth: a systematic review. World J Dent 2024; 15 (05) 452
    Search in Google Scholar
  • 3 Acharya S, Gurunathan D, Assiry AA, Luke AM, Shetty KP, Karobari MI. Comparison of modified NeoPutty MTA®, biodentine, and calcium hydroxide in indirect pulp therapy in deciduous teeth: an in vivo clinical study. Int J Clin Pediatr Dent 2024; 17 (09) 1025-1029
    CrossrefPubMedSearch in Google Scholar
  • 4 Lakshmanan L, Gurunathan D, Shanmugam R. Effectiveness of white tea-mediated silver nanoparticles as an intracanal irrigant against Enterococcus faecalis: an in vitro study. Dent Med Probl 2024; 61 (04) 593-598
    CrossrefPubMedSearch in Google Scholar
  • 5 Tronstad L. Recent development in endodontic research. Scand J Dent Res 1992; 100 (01) 52-59
    PubMedSearch in Google Scholar
  • 6 Goerig AC, Camp JH. Root canal treatment in primary teeth: a review. Pediatr Dent 1983; 5 (01) 33-37
    PubMedSearch in Google Scholar
  • 7 Garcia-Godoy F. Evaluation of an iodoform paste in root canal therapy for infected primary teeth. ASDC J Dent Child 1987; 54 (01) 30-34
    PubMedSearch in Google Scholar
  • 8 Mekkriangkrai D, Nakornchai S, Jirarattanasopha V. Success rate and related factors of Vitapex pulpectomy in primary teeth: a retrospective study. Eur J Dent 2023; 17 (04) 1163-1169
    Thieme ConnectPubMedSearch in Google Scholar
  • 9 Nurko C, Ranly DM, García-Godoy F, Lakshmyya KN. Resorption of a calcium hydroxide/iodoform paste (Vitapex) in root canal therapy for primary teeth: a case report. Pediatr Dent 2000; 22 (06) 517-520
    PubMedSearch in Google Scholar
  • 10 Spedding RH. Incomplete resorption of resorbable zinc oxide root canal fillings in primary teeth: report of two cases. ASDC J Dent Child 1985; 52 (03) 214-216
    PubMedSearch in Google Scholar
  • 11 Hendry JA, Jeansonne BG, Dummett Jr CO, Burrell W. Comparison of calcium hydroxide and zinc oxide and eugenol pulpectomies in primary teeth of dogs. Oral Surg Oral Med Oral Pathol 1982; 54 (04) 445-451
    CrossrefPubMedSearch in Google Scholar
  • 12 Govindaraju L, Jeevanandan G. Evaluation of the antimicrobial efficacy of different concentrations of a novel root canal filling material for primary teeth - an in vitro study. Dent Res J (Isfahan) 2023; 20: 20
    CrossrefPubMedSearch in Google Scholar
  • 13 Govindaraju L, Jeevanandan G. Comparative evaluation of the antimicrobial efficacy of different concentrations of the novel root canal filling material for primary teeth: a microbiological study. J Int Oral Health 2023; 15: 84-88
    CrossrefSearch in Google Scholar
  • 14 Govindaraju L, Jeevanandan G. Antimicrobial efficacy of a novel obturating material used in primary teeth - an in vitro study. J Popul Ther Clin Pharmacol 2023; 30 (06) e149-e156
    Search in Google Scholar
  • 15 Govindaraju L, Jeevanandan G, Veeraraghavan VP. et al. An in-vitro analysis of the antimicrobial efficacy of a novel obturating material for primary teeth. J Int Dent Med Res 2024; 17 (01) 110-114
    Search in Google Scholar
  • 16 Baraba A, Zelježić D, Kopjar N, Mladinić M, Anić I, Miletić I. Evaluation of cytotoxic and genotoxic effects of two resin-based root-canal sealers and their components on human leucocytes in vitro. Int Endod J 2011; 44 (07) 652-661
    CrossrefPubMedSearch in Google Scholar
  • 17 Govindaraju L, Jeevanandan G. Development of an advanced material for obturation in primary teeth based on the microbiological analysis of the antimicrobial efficacy of various concentrations: an in vitro study. Int J Clin Pediatr Dent 2023; 16 (06) 800-803
    PubMedSearch in Google Scholar
  • 18 Teixeira TF, da Silva AM, de Carvalho Coutinho TM. et al. Deciduous molars complexity anatomy reveled by computed microtomography. Eur J Dent 2024; 18 (03) 789-795
    Thieme ConnectPubMedSearch in Google Scholar
  • 19 Nair AV, Nayak M, Prasada LK, Shetty V, Kumar CNV, Nair RR. Comparative evaluation of cytotoxicity and genotoxicity of two bioceramic sealers on fibroblast cell line: an in vitro study. J Contemp Dent Pract 2018; 19 (06) 656-661
    CrossrefPubMedSearch in Google Scholar
  • 20 Dahl JE, Frangou-Polyzois MJ, Polyzois GL. In vitro biocompatibility of denture relining materials. Gerodontology 2006; 23 (01) 17-22
    CrossrefPubMedSearch in Google Scholar