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CC BY 4.0 · Eur J Dent
DOI: 10.1055/s-0045-1802947
Original Article

Zeolite-Y-Loaded Chitosan Nanoparticles as Endodontic Antimicrobial Agent: An In vitro Study

Amir Isam Omer Ibrahim 
1   Department of Restorative Dental Sciences, College of Dentistry, King Faisal University, Al-Ahsa, Saudi Arabia
2   Department of Restorative Dentistry, Faculty of Dentistry, University of the Western Cape, Cape Town, South Africa
,
Desigar Moodley
2   Department of Restorative Dentistry, Faculty of Dentistry, University of the Western Cape, Cape Town, South Africa
3   Edelweiss Dentistry Products Gmbh, Wolfurt, Austria
,
Ernest Maboza
4   WHO Collaborating Centre for Oral Health, Faculty of Dentistry, University of the Western Cape, Cape Town, South Africa
,
Annette Olivier
4   WHO Collaborating Centre for Oral Health, Faculty of Dentistry, University of the Western Cape, Cape Town, South Africa
,
Leslie Petrik
5   Department of Chemistry, Faculty of Natural Sciences, University of the Western Cape, Cape Town, South Africa
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Abstract

Objectives The objectives were to synthesize a bioactive nanocomposite as an endodontic antimicrobial agent by loading previously synthesized electrosprayed chitosan nanoparticles (Ch-Np) into Zeolite-Y as a carrier and compare its antimicrobial activity against two endodontic pathogens using the agar diffusion test. Additionally, the effect of tissue inhibitors (dentin powder and serum albumin) on the antimicrobial activity of the Ch-Np-Zeolite nanocomposite was studied. Finally, the possible cytotoxicity of the novel nanocomposite against Balb/c 3T3 mouse fibroblast cells was evaluated.

Materials and Methods A concentration of 3% (w/v) electrosprayed Ch-Np was mixed with Zeolite-Y in a concentration of 53.3 (w/v) and characterized using high-resolution scanning electron microscopy (HR-SEM) and energy-dispersive X-ray spectroscopy (EDS) analysis. The antimicrobial activity was evaluated against Streptococcus mutans and Enterococcus faecalis using the agar diffusion test, and the time-kill test was performed using the broth microdilution technique in the presence of tissue inhibitors. The cytotoxicity was evaluated against 3T3 mouse fibroblast cells using the standard 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.

Statistical Analysis The difference between the antimicrobial activity of Ch-Np-Zeolite nanocomposite against S. mutans and E. faecalis was analyzed using the Mann–Whitney's U test. The effect of tissue inhibitors on the antimicrobial activity of Ch-Np-Zeolite nanocomposite was analyzed by comparing the mean of log colony-forming unit per milliliter over time. For the cytotoxicity assay, a statistically significant difference between each group and their control was made using a t-test with a probability value of p ≤ 0.05, considered a significant difference.

Results HR-SEM of the dried paste-like mixture Ch-Np-Zeolite revealed the typical crystal habit of the supporting zeolite, and EDS analysis confirmed that the zeolite parent material retained its elemental composition after loading with Ch-Np. The antimicrobial activity of Ch-Np-Zeolite was demonstrated by the mean diameter inhibition zones of 9.57 and 7.85 mm for S. mutans and E. faecalis, respectively. Streptococcus mutans and E. faecalis were completely eradicated in the presence of tissue inhibitors. The Ch-Np-Zeolite nanocomposite significantly promoted the growth of 3T3 fibroblast cells (p < 0.05), supporting its lack of cytotoxicity.

Conclusion Zeolite-Y-loaded Ch-Np nanocomposite shows promising antimicrobial activity while maintaining its biocompatibility even in the presence of tissue inhibitors.

Keywords

chitosan nanoparticles - intracanal medicament - antimicrobial - zeolite - cytotoxicity

Supplementary Material



Publikationsverlauf

Artikel online veröffentlicht:
23. April 2025

© 2025. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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  • References

  • 1 Arias A, Peters OA. Present status and future directions: canal shaping. Int Endod J 2022; 55 (Suppl. 03) 637-655
    PubMedSuche in Google Scholar
  • 2 Jayakkodi H, Reddy A, Krishnan V, Arora S, Cv RR, Joseph S. Assessment of antimicrobial activity of different intracanal medicaments against Enterococcus faecalis and Candida albicans: an in vitro study. J Contemp Dent Pract 2019; 20 (02) 179-183
    PubMedSuche in Google Scholar
  • 3 Ordinola-Zapata R, Noblett WC, Perez-Ron A, Ye Z, Vera J. Present status and future directions of intracanal medicaments. Int Endod J 2022; 55 (Suppl. 03) 613-636
    CrossrefPubMedSuche in Google Scholar
  • 4 Haapasalo M, Qian W, Portenier I, Waltimo T. Effects of dentin on the antimicrobial properties of endodontic medicaments. J Endod 2007; 33 (08) 917-925
    PubMedSuche in Google Scholar
  • 5 Valera MC, Oliveira SA, Maekawa LE. et al. Action of chlorhexidine, Zingiber officinale, and calcium hydroxide on Candida albicans, Enterococcus faecalis, Escherichia coli, and endotoxin in the root canals. J Contemp Dent Pract 2016; 17 (02) 114-118
    PubMedSuche in Google Scholar
  • 6 Chockattu SJ, Deepak BS, Goud KM. Comparison of anti-bacterial efficiency of ibuprofen, diclofenac, and calcium hydroxide against Enterococcus faecalis in an endodontic model: an in vitro study. J Conserv Dent 2018; 21 (01) 80-84
    PubMedSuche in Google Scholar
  • 7 Balasubramaniam R, Jayakumar S. Antibiotics in endodontics-a concise review. Int J Appl Dent Sci 2017; 3 (04) 323-329
    Suche in Google Scholar
  • 8 Janani K, Teja KV, Ajitha P, Sandhya R. Evaluation of tissue inflammatory response of four intracanal medicament - an animal study. J Conserv Dent 2020; 23 (03) 216-220
    PubMedSuche in Google Scholar
  • 9 Makandar SD, Noorani TY. Triple antibiotic paste––challenging intracanal medicament: a systematic review. J Int Oral Health 2020; 12 (03) 189-196
    CrossrefSuche in Google Scholar
  • 10 Gomes BPFA, Vianna ME, Zaia AA, Almeida JFA, Souza-Filho FJ, Ferraz CCR. Chlorhexidine in endodontics. Braz Dent J 2013; 24 (02) 89-102
    CrossrefPubMedSuche in Google Scholar
  • 11 Ibrahim A, Moodley D, Uche C, Maboza E, Olivier A, Petrik L. Antimicrobial and cytotoxic activity of electrosprayed chitosan nanoparticles against endodontic pathogens and Balb/c 3T3 fibroblast cells. Sci Rep 2021; 11 (01) 24487
    PubMedSuche in Google Scholar
  • 12 Gougazeh M, Buhl JC. Synthesis and characterization of zeolite A by hydrothermal transformation of natural Jordanian kaolin. J Assoc Arab Univ Basic Appl Sci 2014; 15 (01) 35-42
    Suche in Google Scholar
  • 13 Laurino C, Palmieri B. Zeolite:“the magic stone”; main nutritional, environmental, experimental and clinical fields of application. Nutr Hosp 2015; 32 (02) 573-581
    PubMedSuche in Google Scholar
  • 14 Dusselier M, Davis ME. Small-pore zeolites: synthesis and catalysis. Chem Rev 2018; 118 (11) 5265-5329
    PubMedSuche in Google Scholar
  • 15 Talebi J, Halladj R, Askari S. Sonochemical synthesis of silver nanoparticles in Y-zeolite substrate. J Mater Sci 2010; 45 (12) 3318-3324
    Suche in Google Scholar
  • 16 Correia BL, Gomes ATPC, Noites R, Ferreira JMF, Duarte AS. New and efficient bioactive glass compositions for controlling endodontic pathogens. Nanomaterials (Basel) 2022; 12 (09) 1577
    PubMedSuche in Google Scholar
  • 17 Haapasalo HK, Sirén EK, Waltimo TM, Ørstavik D, Haapasalo MP. Inactivation of local root canal medicaments by dentine: an in vitro study. Int Endod J 2000; 33 (02) 126-131
    PubMedSuche in Google Scholar
  • 18 Portenier I, Haapasalo H, Rye A, Waltimo T, Ørstavik D, Haapasalo M. Inactivation of root canal medicaments by dentine, hydroxylapatite and bovine serum albumin. Int Endod J 2001; 34 (03) 184-188
    PubMedSuche in Google Scholar
  • 19 Sutton S. Accuracy of plate counts. J Valid Technol 2011; 17 (03) 42-46
    Suche in Google Scholar
  • 20 Roberta T, Federico M, Federica B, Antonietta CM, Sergio B, Ugo C. Study of the potential cytotoxicity of dental impression materials. Toxicol In Vitro 2003; 17 (5-6): 657-662
    PubMedSuche in Google Scholar
  • 21 Hassan R, Khallaf M. Effect of a silver nanoparticle intracanal-based medicament on the microhardness of human radicular dentine. Endo (New Malden) 2018; 12 (02) 125-131
    Suche in Google Scholar
  • 22 Geurtsen W, Leinenbach F, Krage T, Leyhausen G. Cytotoxicity of four root canal sealers in permanent 3T3 cells and primary human periodontal ligament fibroblast cultures. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998; 85 (05) 592-597
    PubMedSuche in Google Scholar
  • 23 Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65 (1-2): 55-63
    CrossrefPubMedSuche in Google Scholar
  • 24 Grobler SR, Oliver A, Moodley D, Van Wyk Kotze TJ. Cytotoxicity of recent dentin bonding agents on mouse fibroblast cells. Quintessence Int 2008; 39 (06) 511-516
    PubMedSuche in Google Scholar
  • 25 Lutz W, Zeolite Y. Synthesis, modification, and properties—a case revisited. Adv Mater Sci Eng 2014; 2014 (01) 724248-724268
    Suche in Google Scholar
  • 26 Taufiqurrahmi N, Mohamed AR, Bhatia S. Nanocrystalline zeolite Y: synthesis and characterization. IOP Conf Ser: Mater Sci Eng 2011; 17 (01) 012030
    Suche in Google Scholar
  • 27 Narayanan LL, Vaishnavi C. Endodontic microbiology. J Conserv Dent 2010; 13 (04) 233-239
    CrossrefPubMedSuche in Google Scholar
  • 28 Lima AR, Herrera DR, Francisco PA. et al. Detection of Streptococcus mutans in symptomatic and asymptomatic infected root canals. Clin Oral Investig 2021; 25 (06) 3535-3542
    PubMedSuche in Google Scholar
  • 29 Hardie JM, Whiley RA. Classification and overview of the genera Streptococcus and Enterococcus . J Appl Microbiol 1997; 83 (S1): 1S-11S
    PubMedSuche in Google Scholar
  • 30 Schleifer KH, Kilpper-Bälz R. Transfer of Streptococcus faecalis and Streptococcus faecium to the genus Enterococcus nom. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov. Int J Syst Evol Microbiol 1984; 34 (01) 31-34
    Suche in Google Scholar
  • 31 Chandrasekaran M, Kim KD, Chun SC. Antibacterial activity of chitosan nanoparticles: a review. Processes (Basel) 2020; 8 (09) 1173
    Suche in Google Scholar