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CC BY 4.0 · Eur J Dent
DOI: 10.1055/s-0045-1806950
Original Article

7F2 Preosteoblast Cell Line Relative Viability Percentage after the Administration of 1% Roselle Flower Extract Nanoemulsion (Hibiscus sabdariffa Linn.)

Ridhofar Akbar Khusnul Abdillah
1   Dental Health Science Study Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Nadya Rafika Amalia
1   Dental Health Science Study Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Theresia Indah Budhy
2   Department of Oral and Maxillofacial Pathology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Muhammad Luthfi
3   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Rini Devijanti Ridwan
3   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Devi Rianti
4   Department of Dental Material, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Taufan Bramantoro
5   Department of Dental Public Health, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Nastiti Faradilla Ramadhani
6   Department of Dentomaxillofacial Radiology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Ida Bagus Narmada
7   Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Putri Cahaya Situmorang
8   Study Program of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia
,
Khairul Anuar Shariff 
9   Department of Materials Engineering, School of Materials and Mineral Resources Engineering, Engineering Campus, Universiti Sains Malaysia, Penang, Malaysia
,
Tengku Natasha Eleena binti Tengku Ahmad Noor
10   Dentistry Division, 609 Armed Force Dental Clinic, Sarawak, Malaysia
,
Alexander Patera Nugraha
7   Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
› Institutsangaben
Funding This study received a Penelitian Tesis Magister research grant in 2024 (DRTPM) from the Ministry of Education, Republic of Indonesia.
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Abstract

Objective

The roselle flower (Hibiscus sabdariffa) has shown potential as an alternative therapy for bone regeneration. This flower extract can induce osteoblast maturation, which is crucial for forming new bone. The study aim was to evaluate the viability of the 7F2 preosteoblast cell line following the application of a roselle flower nanoemulsion extract (RNE).

Materials and Methods

This study utilized the 7F2 preosteoblast cell line to assess cell viability (%). The RNE was oven-dried at 35 to 40°C for 6 hours, resulting in a solid extract. The extract was then diluted into different concentrations. The preparation of 1% RNE was stirred at 1,400 rpm at 50°C for 90 minutes. Primary cultures of preosteoblast cell lines (7F2 cells) were distributed across 10 wells. Well 1 served as the positive control, representing 100% cell viability. Well 2 acted as the media control, containing only culture media without cells, representing 0% cell viability. Wells 3 to 10 were exposed to 1% RNE at serial concentrations of 100, 50, 25, 12.5, 6.25, 3.125, 1.56, and 0.78%. The viability of the 7F2 preosteoblast cell line was assessed using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide or microtetrazolium assay. The treatment was conducted on days 1, 3, and 7 for observation.

Results

The findings indicated that the highest cell viability was observed on day 7, averaging 89.27% at a 0.78% concentration, while the lowest viability was 2.60% at a 100% concentration.

Conclusion

These results suggest that RNE is nontoxic to the 7F2 preosteoblast cell line.

Keywords

Hibiscus sabdariffa - preosteoblast - medicine - orthodontics - viability

Data Availability Statement

Derived data supporting the findings of this study are available from the corresponding author upon request.


Ethical Approval Statement

The research will be conducted at the Dental Research Center, Faculty of Dentistry, Universitas Airlangga, Surabaya, from February to May 2024. This research is ethically cleared at Airlangga University with number 0652/HRECC.FODM/VII/2024.




Publikationsverlauf

Artikel online veröffentlicht:
01. Mai 2025

© 2025. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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