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CC BY 4.0 · European Journal of General Dentistry
DOI: 10.1055/s-0045-1811202
Original Article

Evaluating the Oral Wound Healing Properties and Antimicrobial Efficacy of Sulfonated Shrimp Chitosan

Rachmi Fanani Hakim 
1   Department of Oral Biology, Faculty of Dentistry, Syiah Kuala University, Banda Aceh, Indonesia
,
Fakhrurrazi Fakhrurrazi
2   Department of Maxillofacial Surgery, Faculty of Dentistry, Syiah Kuala University, Banda Aceh, Indonesia
,
Anisa Deria
3   Faculty of Dentistry, Syiah Kuala University, Banda Aceh, Indonesia
,
Nindytha Halidzya Feba
3   Faculty of Dentistry, Syiah Kuala University, Banda Aceh, Indonesia
,
Siti Lola Istiqamah
3   Faculty of Dentistry, Syiah Kuala University, Banda Aceh, Indonesia
,
Nabilah Amalia
3   Faculty of Dentistry, Syiah Kuala University, Banda Aceh, Indonesia
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Abstract

Objective

This study evaluated the oral wound healing efficacy and fibroblast proliferation induced by sulfonated Vannamei shrimp chitosan, compared with commercial chitosan. Additionally, its antimicrobial activity against oral microorganism Streptococcus mutans, Staphylococcus aureus, Aggregatibacter actinomycetemcomitans, and Porphyromonas gingivalis was assessed.

Materials and Methods

In the wound healing study, three groups of rats were treated with Aquades (negative control), commercial chitosan (positive control), and sulfonated shrimp chitosan, with healing time and fibroblast proliferation measured. The antimicrobial study tested different concentrations (1, 3, 5, and 7%) using a disk diffusion method.

Results

Sulfonated shrimp chitosan showed the fastest wound healing (5 ± 0.47 days) compared with commercial chitosan (6 ± 0.0 days) and Aquades (6.6 ± 0.6 days) (p = 0.036), and significantly higher fibroblast counts (20.32 ± 0.688, p < 0.001). Antimicrobial activity was moderate, with the highest inhibition at 3 or 5%. The ANOVA (analysis of variance) test was used for hypothesis testing with the significance level at p <0.05, followed by a Kruskal–Wallis test.

Discussion

Sulfonated chitosan demonstrated superior wound healing properties, consistent with studies showing enhanced tissue regeneration by sulfonated chitosan derivatives. Antimicrobial effects were moderate, with peak activity at intermediate concentrations and a decrease at 7%, possibly due to a saturation effect.

Conclusion

These findings suggest sulfonated shrimp chitosan could be a promising agent in wound healing and infection control.

Keywords

sulfonated chitosan - antimicrobial activity - wound healing


Publication History

Article published online:
08 September 2025

© 2025. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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