Abstract
Objective
A new type of platelet concentrate used in regenerative periodontal therapy, called
concentrated platelet-rich fibrin (C-PRF), contains more growth factors than previous
generations of platelet concentrate. Compared to fixed-angle centrifugation, a recently
discovered technique called horizontal centrifugation can produce C-PRF with a more
evenly distributed quantity of platelets and improved biological properties. This
study aims to determine the effect of horizontal and fixed-angle centrifugation methods
on the release of transforming growth factor β1 (TGF-β1) and platelet-derived growth
factor AB (PDGF-AB), as well as on the migration and proliferation of osteoblast cells.
Materials and Methods
This study used two test groups: a horizontal group and a fixed-angle group. The release
of growth factors TGF-β1 and PDGF-AB was measured at five time points: the first,
third, seventh, 10th, and 14th days of incubation, using the enzyme-linked immunosorbent assay. To determine
the effect of C-PRF type on osteoblast cell migration, a scratch wound healing assay
was conducted 24 hours after incubation. Osteoblast cell proliferation was counted
using a 450-nm microplate reader to determine the number of cells in the well plate
on the first, third, and fifth days, with the Cell Counting Kit-8. The data were examined
using two-way ANOVA (analysis of variance) and post hoc LSD (least significant difference).
Results
The findings indicated a statistically significant increase in the release of TGF-β1
and PDGF-AB in the horizontal group compared to the fixed-angle group over a 14-day
period. The horizontal group's TGF-β1 release increased by a factor of 1.5 compared
to the fixed-angle group, peaking on day 7. Following a 24-hour incubation period
and microscopic examination, the horizontal group exhibited greater osteoblast migratory
activity than the control group and the fixed-angle centrifugation group. Horizontal
centrifugation C-PRF had a significantly greater effect on osteoblast proliferation
than fixed-angle centrifugation. The horizontal group's proliferation rate surpassed
that of the fixed-angle group by up to 1.5 times on the third day of observation.
Conclusion
Compared to fixed-angle centrifugation, the horizontal method of preparing C-PRF has
a greater effect on the release of growth factors and the behavior of osteoblasts.
Keywords
horizontal centrifugation - fixed-angle centrifugation - concentrated platelet-rich
fibrin - TGF-β1 - PDGF-AB - proliferation - migration
