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Int J Sports Med 2000; 21(8): 561-565
DOI: 10.1055/s-2000-8485
Physiology and Biochemistry
Georg Thieme Verlag Stuttgart · New York

Loss of Myocardial CK-MB into the Circulation Following 3.5 Hours of Swimming in a Rat Model

Y. J. Chen1 , R. C. Serfass1 , F. S. Apple1, 2
  • 1 Division of Kinesiology, School of Kinesiology and Leisure Studies, University of Minnesota, USA
  • 2 Department of Laboratory Medicine and Pathology, Hennepin County Medical Center, University of Minnesota School of Medicine, Minneapolis, MN, USA
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Publication History

Publication Date:
31 December 2000 (online)

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The purpose of this study was to document alterations of creatine kinase-B (CK-B) in the left and right ventricles of rats and CK-MB release into the circulation following a single bout of stressful prolonged intense exercise. Male Sprague-Dawley rats, with 8 % bodyweight attached to each tail, were forced to swim 3.5 hours and were then sacrificed immediately (0 h PS), 3 hours (3 h PS), 24 hours (24 h PS), and 48 hours (48 h PS) post swimming, respectively. Sedentary (control) rats were sacrificed at rest. Serum CK-MB mass increased 2.1 times (8.9 µg/L; p < 0.01 vs. controls of 4.3 µg/L) and 1.4 times (6.0 µg/L; P < 0.01 vs. controls) at 0 h PS, and 3 h PS, respectively, and returned to baseline at 24 h PS. Western blot analysis indicated that CK-B of the right ventricle decreased 14 % (p < 0.05), 20 % (p < 0.01), and 12 % (p < 0.05) at 3 h PS, 24 h PS and 48 h PS, respectively. The CK-B of the left ventricles decreased 34 % (p < 0.05) at 0 h PS, returned to baseline at 3 h PS, and was increased 39 % (P < 0.01) at 48 h PS. Our findings demonstrate that a single bout of stressful, prolonged, intense exercise resulted in CK-B subunit loss from the myocardium, resulting in increased serum CK-MB concentrations, an indication of myocardial injury.

Key words:

Myocardial injury, exercise, creatine kinase isoenzymes.

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Ph. D. F. S. Apple

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701 Park Ave. Minneapolis, MN 55415 USA

Phone: Phone:+ 1 (612) 3473324

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