An Exploratory Study into the Effect of Exhausting Bicycle Exercise on Endocrine and Immune Responses in Post-Menopausal Women: Relationships between Vigour and Plasma Cortisol Concentrations and Lymphocyte Proliferation following Exercise

It is well-established that bicycle exercise alters the endocrine and immune responses in men, but little information is available for women, especially middle-aged, post-menopausal women. The purpose of our study was to document the endocrine and immune reactivity to exhausting bicycle exercise in post-menopausal women, and to explore whether complaints of fatigue or low vigour are related to these exercise-induced responses. Thirteen healthy post-menopausal women participated in this study. We used a graded exercise protocol to study the kinetics of activation of the endocrine and immune system. We chose to examine hormones related to the hypothalamus-pituitary-adrenal (HPA) system such as adrenocorticotropin hormone (ACTH) and cortisol and hormones related to the pituitary such as prolactin (PRL) and growth hormone (GH). With regard to the immune system, we examined the natural killer (NK) cell activity and pokeweed (PWM)-induced lymphocyte proliferation in addition to changes in peripheral blood cell counts. Our results demonstrate that acute physical stress results in a strong release of ACTH, cortisol, GH and PRL. The bicycle test significantly increased the number of CD3⁺, CD4⁺, CD16/56⁺ (NK cells) and CD8⁺ cells in our group of post-menopausal women. Interestingly, NK activity did not increase significantly despite an increase in NK cell numbers. PWM-induced lymphocyte proliferation did not change either. In addition, our data support the hypothesis that low vigour in post-menopausal women interferes with the endocrine and immune responses to exhausting exercise. In women with complaints of low vigour we found lower cortisol responses and higher increments in the proliferative capacity of lymphocytes as compared to those with high vigour scores. NK activity was unrelated to exhaustive mood states. These data indicate that endocrine as well as immune system activity changes in response to exhausting exercise in middle-aged, post-menopausal women. In addition, exhaustive mood states may contribute to cortisol responses and function of peripheral immune cells in post-menopausal women following exhausting exercise.

Key words:

Bicycle exercise - HPA system - immune system - vigour - post-menopausal women.

References

• 1 Benschop R J, Nieuwenhuis E ES, Tromp E AM, Godaert G LR, Ballieux R E, Van Doornen L JP. Effects of ß-adrenergic blockade on immunologic and cardiovascular changes induced by mental stress.  Circulation. 1994;  89 762-769
• 2 Chearskul S, Srichantaap T. Hormonal and metabolic responses to acute exercise in Thai women.  J Med Assoc Thai. 1994;  77 400-409
• 3 Coiro V, Volpi R, Maffei M L, Caiazza A, Caffarri G, Capretti L, Colla R, Chiodera P. Opioid modulation of gamma-aminobutyric acid-controlled inhibition of exercise-stimulated growth hormone and prolactin secretion in normal men.  Eur J Endocrinol. 1994;  131 50-55
• 4 Fiatarone M A, Morley J E, Bloom E T, Benton D, Solomon G F, Makinodam T. The effect of exercise on natural killer cell activity in young and old subjects.  J Gerontol Med Sci. 1989;  44 M37-M45
• 5 Fischer H G, Hollman W, De Meirleir K. Exercise changes in plasma tryptophan fractions and relationship with prolactin.  Int J Sports Med. 1991;  12 487-489
• 6 Gray A B, Smart Y C, Telford R D, Weideman M J, Roberts T K. Anaerobic exercise causes transient changes in leukocyte subsets and IL-2R expression.  Med Sci Sports Exerc. 1992;  24 1332-1338
• 7 Hyyppa M T, Aunola S, Kuusela V. Psychoendocrine responses to bicycle exercise in healthy ment in good physical condition.  Int J Sports Med. 1986;  7 89-93
• 8 Johannessen A, Hagen C, Galbo H. Prolactin, growth hormone, thyrotropin, 3,5,3’-triiodothyronine, and thyroxine responses to exercise alter fat- and carbohydrate-enriched diet.  J Clin Endocrinol Metab. 1981;  52 56-61
• 9 Kappel M, Tvede N, Galbo H, Haahr P M, Kjaer M, Linstow M, Klarlund K, Pedersen B K. Evidence that the effect of physical exercise on NK cell activity is mediated by epinephrine.  J Appl Physiol. 1991;  70 2530-2534
• 10 McNair D M, Lorr M, Droppleman G J. Profile of Mood States. San Diego, California; Educational and Industrial Testing Service 1971
• 11 Meirleir K d e, Naaktgeboren N, Steirteghem A v an, Gorus F, Olbrecht J, Block P. Beta-endorphin and ACTH levels in peripheral blood during and after aerobic and anaerobic exercise.  Eur J Appl Physiol. 1986;  55 5-8
• 12 Nielsen H B, Pedersen B K. Lymphocyte proliferation in response to exercise.  Eur J Appl Physiol. 1997;  75 375-379
• 13 O’Connor P J, Corrigan D L. Influence of short-term cycling on salivary cortisol levels.  Med Sci Sports Exerc. 1987;  19 224-228
• 14 Odagiri Y, Shimomitsu T, Iwane H, Katsumura T. Relationships between exhaustive mood state and changes in stress hormones following an ultraendurance race.  Int J Sports Med. 1996;  17 325-331
• 15 Odink J, Beek EJ van d er, Berg H van d en, Bogaards J JP, Thissen J TNM. Effect of work load on free and sulfate-conjugated plasma catecholamines, prolactin and cortisol.  Int J Sports Med. 1986;  7 352-357
• 16 Odio M, Brodish A. Age-related adaptation of pituitary-adrenocortical responses to stress.  Neuroendocinology. 1989;  49 382-388
• 17 Opstad P K. Alterations in the morning plasma levels of hormones and the endocrine responses to bicycle exercise during prolonged strain. The significance of energy and sleep deprivation.  Acta Endocrinologica. 1991;  125 14-22
• 18 Pedersen B K. Influence of physical activity on the cellular immune system: Mechanisms of action.  Int J Sports Med. 1991;  12 S23-S29
• 19 Pedersen B K, Tvede N, Hansen F R, Andersen V, Bendix T, Bendixen G, Bendtzen K, Galbo H, Haahr P M, Klarlund K, Sylvest J, Thomsen B S, Halkjaer-Kristensen J. Modulation of natural killer cell activity in peripheral blood by physical exercise.  Scand J Immunol. 1988;  27 673-678
• 20 Sapolsky R M, Krey L C, McEwen B S. The neuroendocrinology of stress and aging: the glucocorticoid cascade hypothesis.  Endocr Rev. 1986;  7 284-301
• 21 Schedlowski M, Hosch W, Oberbeck R, Benschop R J, Jacobs R, Raab H R, Schmidt R E. Catecholamines modulate human NK cell circulation and function via spleen-independent beta 2-adrenergic mechanisms.  J Immunol. 1996;  156 93-99
• 22 Shek P N, Sabistan B H, Buguet A, Radomski M W. Strenuous exercise and immunological changes: A multiple-time-point analysis of leukocyte subsets, CD4/CD8 ratio, immunoglobulin production and NK cell response.  Int J Sports Med. 1995;  16 466-474
• 23 Shinkai S, Shore S, Shek P N, Shephard R J. Acute exercise and immune function. Relationship between lymphocyte activity and changes in subset counts.  Int J Sports Med. 1992;  13 452-461
• 24 Tvede N, Pedersen B K, Hansen F R, Bendix T, Christensen L D, Galbo H, Halkjaer-Kristensen J. Effect of physical exercise on blood mononuclear cell subpopulations and in vitro proliferative responses.  Scand J Immunol. 1989;  29 383-389
• 25 Tvede N, Kappel M, Halkjaer-Kristensen J, Galbo H, Pedersen B K. The effect of light, moderate and severe bicycle exercise on lymphocyte subsets, natural killer and lymphokine activated killer cells, lymphocyte proliferative response and interleukin 2 production.  Int J Sports Med. 1993;  14 275-282
• 26 Wald F DM, Mellenberg G J. De verkorte versie van de Nederlandse vertaling van de Profile of Mood States (POMS).  Ned Tijdschr Psychol. 1990;  45 86-99