Horm Metab Res 2001; 33(12): 701-707
DOI: 10.1055/s-2001-19140
Original Basic
© Georg Thieme Verlag Stuttgart · New York

In Vitro and In Vivo Impairment of α2-Adrenergic Receptor-Dependent Antilipolysis by Fatty Acids in Human Adipose Tissue

S. Gesta 1 , J. Hejnova 2 , M. Berlan 1 3 , D. Daviaud 1 , F. Crampes 1 4 , V. Stich 2 , P. Valet 1 , J.-S. Saulnier-Blache 1
  • 1 INSERM U317, Rangueil Hospital, Paul Sabatier University, Toulouse, France
  • 2 Department of Sport Medicine, Third Faculty of Medicine, Charles University, Prague, Czech Republic
  • 3 Department of Medical and Clinical Pharmacology, Purpan Faculty of Medicine, Toulouse, France
  • 4 Department of Adaptation to Exercise, Purpan Hospital, Toulouse, France
Further Information

Publication History

Publication Date:
18 December 2001 (online)

The aim of the present study was to study the influence of fatty acids on the adrenergic control of lipolysis both in vitro and in vivo. Human subcutaneous adipose tissue explants were cultured for 48 h in the presence of 100 µM bromopalmitate (BrPal), and lipolysis was measured in isolated adipocytes. In control conditions, β-AR-dependent activation of lipolysis by epinephrine was almost undetectable, and could be fully restored by pharmacological blockade of α2-AR-dependent antilipolysis. After BrPal treatment, epinephrine became fully lipolytic and was no longer influenced by α2-AR-blockade. Radioligand binding analysis revealed that BrPal treatment led to a significant reduction in the coupling of α2-AR to G proteins. In parallel, a chronic and significant increase in plasma fatty acids resulting from a 4-day high-fat diet (HFD) was accompanied by an impairment of the amplifying effect of the α2-AR antagonist phentolamine on exercise-induced lipolysis (measured in the subcutaneous adipose tissue with the use of a microdialysis probe) normally observed after a low-fat diet. In conclusion, in vitro and in vivo studies showed that fatty acids impair α2-AR-dependent antilipolysis.


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Jean-Sebastien Saulnier-Blache

INSERM U317, CHU Ranguiel
Université de Paul Sabatier

31403 Toulouse Cédex 4

Phone: + 33 (5) 62 17 29 56

Fax: + 33 (5) 61 33 17 21

Email: saulnier@toulouse.inserm.fr