Facial plast Surg 2002; 18(1): 059-068
DOI: 10.1055/s-2002-19828
Copyright © 2002 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New York, NY 10001, USA. Tel.: +1(212) 584-4662

Tissue Engineering with Chondrocytes

R. James Koch, Goutham Krishna Gorti
  • Division of Otolaryngology-Head and Neck Surgery, Stanford University Medical Center, Stanford, CA
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Publication History

Publication Date:
29 January 2002 (online)

ABSTRACT

Tissue engineering of cartilage, using chondrocytes based on the use of synthetic biodegradable polymer cell delivery vehicles (scaffolds), is an alternate treatment modality for replacing missing cartilage.[1] [2] Cartilage tissue engineering has an important role to play in the generation of graft material for head and neck reconstruction. It is an approach to fabricate cartilage constructs in vitro, which could be used in reconstructive surgery. Methods involve (1) harvesting septal cartilage during septoplasty, (2) isolating chondrocytes through enzymatic digestion of the septal cartilage, (3) expanding the cell number in a two-dimensional monolayer culture, using serum-free media, (4) seeding the cells onto a biodegradable polymer scaffold, and (5) cultivating the seeded scaffolds in a rotating bioreactor. In this article we briefly outline the methodology and clinical applications of cartilage grown ex vivo.