Naive and Memory T Cell Subsets are Differentially Mobilized During Physical Stress

G. A. Gannon; S. Rhind; P. N. Shek; R. J. Shephard

doi:10.1055/s-2002-23173

International Journal of Sports Medicine, Table of Contents

Int J Sports Med 2002; 23(3): 223-229
DOI: 10.1055/s-2002-23173

Immunology

Naive and Memory T Cell Subsets are Differentially Mobilized During Physical Stress

G. A. Gannon^1, ³ , S. Rhind¹ , P. N. Shek¹ , R. J. Shephard^1, ²

¹Defence & Civil Institute of Environmental Medicine, Toronto, ON, Canada
²Faculty of Physical Education & Health, University of Toronto, Toronto, ON, Canada
³Faculty of Physical Education & Recreation Studies, University of Manitoba, Winnipeg, MB, Canada

Abstract

This study examined the naive and memory phenotypic profiles of CD4⁺ and CD8^hi T cells that were mobilized to the peripheral circulation during a combination of aerobic exercise and heat stress, determining expression of the adhesion molecules CD62L and CD11a on the recruited cells. Twelve recreationally active males (age 27.1 ± 5.3 yr, height 1.77 ± 0.08 m, mass 76.9 ± 12.0 kg, V˙O₂peak 43.9 ± 6.7 mL × kg^-1 × min^-1) completed a 40 min bout of cycle ergometry at 65 % of V˙O₂peak while immersed to mid-chest in a water bath at 39 °C. Venous blood samples were collected before (T₀), during (T₄₀) and 30 min after (T₇₀) exposure to combined exercise and heat stress. Specimens were analyzed by three-colour flow cytometry for CD4⁺ and CD8^hi T cell expression of CD45RO, CD11a and CD62L. Some 80 % of the CD4⁺ T cells that were mobilized were of the CD45RO memory phenotype, with the numbers of CD11a^lo and CD62L⁺ cells increasing more than those of CD11a^hi and CD62L^- cells. For the CD8^hi cells, there was a more equal recruitment of CD45RO^- naive (43 %) and CD45RO⁺ memory (57 %) cells. The majority (84 %) of recruited CD8⁺ cells were CD11a^hi; there was a trend to predominance of CD62L^- cells (57 %) for the memory subset, but with almost equal recruitment of CD62L^+/- for the naive subset. We conclude that the exercise + heat stress induced trend to an increase in CD4⁺ T cells is linked in some way to memory phenotype; it cannot be explained simply by a high density expression of CD11a and lack of the lymph node homing receptor (CD62L). Furthermore, although mobilization of CD8^hi T cells is not linked to memory phenotype, a high density expression of CD11a and a lack of the lymph node homing receptor are important determinants of CD8^hi T cell mobilization.

Key words

Adhesion molecules - exercise immunology - lymphocyte mobilization - anti-inflammatory T cells

Full Text

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