Int J Sports Med 2002; 23(3): 202-206
DOI: 10.1055/s-2002-23179
Orthopedics and Clinical Science
© Georg Thieme Verlag Stuttgart · New York

Ultrasound-Guided Percutaneous Biopsies of the Semitendinosus Muscle following ACL Reconstruction - A Methodological Description

A.  Shalabi1 , K.  Eriksson2 , E.  Jansson3 , T.  Wredmark4
  • 1Department of Radiology, Huddinge University Hospital, Karolinska Institutet, Huddinge, Sweden
  • 2Department of Orthopaedic Surgery, Stockholm Söder Hospital, Stockholm, Sweden
  • 3Department of Medical Laboratory Sciences and Technology, Huddinge University Hospital, Karolinska Institutet, Huddinge, Sweden
  • 4Department of Orthopaedic Surgery, Huddinge University Hospital, Karolinska Institutet, Huddinge, Sweden
Further Information

Publication History

August 30, 2001

Publication Date:
26 March 2002 (online)


Purpose: To evaluate whether the ultrasound-guided biopsy technique could be used to analyse the histochemical profile of m. semitendinosus following ACL reconstruction with autologous semitendinosus tendon graft. Material and Methods: Sixteen patients were included in the study. During ACL surgery, specimens from m. semitendinosus were obtained via open biopsy. Ultrasound-guided biopsies from m. semitendinosus were performed 7 - 11 months postoperatively from operated leg (n = 16) and non-operated leg (n = 8). Results: Two biopsy samples were pooled and formed a specimen of approximately 150 fibres. For fibre type composition, there was correlation between open biopsy and ultrasound-guided biopsy data, (P = 0.003, r = 0.89). The r-value for mean fibre area was 0.72 (P = 0.06). The methodological error for fibre type 1 % and mean fibre area was 9 %. Postoperatively, no significant differences were found in fibre type composition or fibre type areas between the operated and non-operated leg. Conclusion: Ultrasound-guided biopsy is an easy and safe technique that can be used for scientific studies and in clinical practice where biopsy from small muscles or from special location in muscles, such as in ruptures, scars or others is needed.


A. Shalabi

Department of Radiology · Karolinska Institutet · Huddinge University Hospital

141 86 Huddinge · Sweden ·

Phone: +46 8 58580812

Fax: +46 8 7114840