Int J Sports Med 2002; 23(8): 595-599
DOI: 10.1055/s-2002-35524
Immunology

© Georg Thieme Verlag Stuttgart · New York

Expression of CD94 and 56bright on Natural Killer Lymphocytes - the Influence of Exercise

P.  L.  Horn1 , K.  Leeman2 , D.  B.  Pyne3 , C.  J.  Gore3
  • 1University of Canberra, Division of Science and Design, ACT, Australia
  • 2Commonwealth Department of Health and Aged Care, ACT, Australia
  • 3Australian Institute of Sport, Australian Sports Commission, ACT, Australia
Further Information

Publication History

Publication Date:
19 November 2002 (online)

Abstract

This study was undertaken to determine the effects of acute intense exercise on the cell membrane-bound glycoprotein designated cluster of differentiation (CD) 94. This marker on natural killer (NK) lymphocytes contributes to control of cell function. CD94 was measured on natural killer lymphocytes from 11 adult (average 25 yrs), well-trained male subjects, (V˙O2peak mean, 5.01 L × min-1) before and immediately after a final, 4 min all-out, cycle ergometry test. Using flow cytometry, lymphocyte populations were distinguished as either having (CD94+) or lacking (CD94-) the cell marker. The absolute number of CD94+ and CD94- natural killer cells increased with exercise but the mean fluorescence intensity (MFI) for CD94 decreased from pre 131, to post 117 (p = 0.01). The percentage of NK cells that were CD94+ did not change, but exercise did mobilise natural killer cells of greater MFI for the surface markers designated CD16/CD56 (pre 750 to post 1 050, p < 0.001). The latter suggests that some exercise-mobilised natural killer cells may have originated from the liver as CD56+bright cells.

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P. L. Horn

University of Canberra · Division of Science and Design

ACT 2601 · Australia ·

Phone: +61 (2) 62012243

Fax: +61 (2) 62015727

Email: horn@scides.canberra.edu.au

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