Saucernetin-7 and Saucernetin-8 Isolated from Saururus chinensis Inhibit the LPS-induced Production of Nitric Oxide and Prostaglandin E2 in Macrophage RAW264.7 Cells

Hee-Juhn Park; Ryung-Gue Kim; Bo-Rim Seo; Joohun Ha; Byung-Tae Ahn; Song-Hae Bok; Yong Sup Lee; Hyoung Ja Kim; Kyung-Tae Lee

doi:10.1055/s-2003-45106

Planta Med 2003; 69(10): 947-950
DOI: 10.1055/s-2003-45106

Letter

Saucernetin-7 and Saucernetin-8 Isolated from Saururus chinensis Inhibit the LPS-induced Production of Nitric Oxide and Prostaglandin E₂ in Macrophage RAW264.7 Cells

Hee-Juhn Park¹ , Ryung-Gue Kim² , Bo-Rim Seo² , Joohun Ha³ , Byung-Tae Ahn⁴ , Song-Hae Bok⁴ , Yong Sup Lee⁵ , Hyoung Ja Kim⁵ , Kyung-Tae Lee²

¹Division of Applied Plant Sciences, Sangji University, Wonju, Korea
²College of Pharmacy, Kyung Hee University, Seoul, Korea
³College of Medicine, Kyung Hee University, Seoul, Korea
⁴Cardiovascular Research laboratory, Korea Research Institute of Bioscience and Biotechnology, Daejon, Korea
⁵Division of Life Sciences, KIST, Seoul, Korea

This research was supported by a grant (PF002104-07) from the Plant Diversity Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology of the Korean Government

Abstract

In the present study, we demonstrate that saucernetin-8 (1) and saucernetin-7 (2), isolated from the underground parts of Saururus chinensis (Saururaceae), exerted potent inhibitory effects on LPS-induced nitric oxide (NO) and prostaglandin E₂ (PGE₂) production in RAW 264.7 cells. Both compounds 1 and 2, known as dineolignans, also suppressed the expression of iNOS and COX-2 protein in a dose-dependent manner. Thus, this study suggests that compounds 1 and 2-mediated inhibition of iNOS and COX-2 expression may be one of the mechanisms responsible for the anti-inflammatory effects of the underground parts of Saururus chinensis.

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References

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Dr. Kyung-Tae Lee

Department of Biochemistry

College of Pharmacy

Kyung-Hee University

Dongdaemun-Ku

Hoegi-Dong 130-701

Seoul

Korea

Phone: +82-2-9610860

Fax: +82-2-9663885

Email: ktlee@khu.ac.kr