Optimization of autologous fibrin gel scaffold for cardiovascular tissue engineering

Objectives Fibrin Gel combines a number of important properties of an ideal scaffold. Degradation and polymerization is controllable, the gel structure allows an homogenous cell distribution with an excellent tissue development. The new developed molding technique allows the production of complex 3-D cardiovascular structures like demonstrate before. The weak point of the fibrin gel scaffold is the low initial mechanical stiffness.

Material and Methods: Different isolation protocols were developed to increase the effectiveness of fibrinogen precipitation of autologous whole blood by a controlled freezing process. Furthermore the fibrin gel composition was modified followed by measurements of the mechanical burst strength. To optimize tissue development the use of different growth factors was studied and qualified by several immunohistochemical investigation regarding the differentiation of the cells and the maturation of the extracellular matrix.

Results: A significant increase of fibrinogen mass were possible by the new isolation protocol. The modified composition of the fibrin gel leads to an increase of the initial burst strength up to 120mmHg. The use of PDGF (Platelet Derived Growth Factor), which can be produced from patient's blood, showed a significant influence on tissue development.

Conclusions: The further development of the fibrin gel matrix leads to an increase of the mechanical properties and tissue development. This make the use of fibrin