Int J Sports Med 2004; 25(7): 522-527
DOI: 10.1055/s-2004-820953
Physiology & Biochemistry

© Georg Thieme Verlag KG Stuttgart · New York

Leukocyte Heat Shock Protein Expression Before and After Intensified Training

M. Whitham1 , 3 , S. L. Halson1 , 2 , G. I. Lancaster1 , M. Gleeson1 , A. E. Jeukendrup1 , A. K. Blannin1
  • 1Human Performance Laboratory, School of Sport and Exercise Sciences, University of Birmingham, Birmingham, U.K.
  • 2School of Human Movement Studies, Queensland University of Technology, Brisbane, Australia
  • 3School of Sport, Health, and Exercise Science, University of Wales, Bangor, Wales, U.K.
Further Information

Publication History

Accepted after revision: October 25, 2003

Publication Date:
26 July 2004 (online)

Abstract

The purpose of the current research was to test the hypothesis that exercise induced leukocyte heat shock protein (HSP) expression is increased during periods of intensified exercise training. Seven male endurance cyclists carried out tests of maximal oxygen consumption and endurance capacity. These standard exercise tests were carried out prior to and following 6 days of prescribed intensified training. Sampled leukocytes were examined for Hsp27 and Hsp70 expression using a Fluorescence Activated Cell Scanner (EPICS XL, Coulter). During a period of overreaching, as signified by a drop in time to fatigue following the intensified training period (p = 0.02), the number of extracellular Hsp27 positive granulocytes increased in response to the VO2max test. Acute, intracellular HSP responses were observed in both baseline and overreached conditions.The present study showed that a period of intensified training caused adaptations in the acute heat shock protein exercise response, reflected by a greater increase of cell surface HSP positive leukocytes following heavy training.

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Dr M. Whitham

School of Sport, Health, and Exercise Science
University of Wales, Bangor, George Building

Holyhead Road

Bangor, Gwynedd LL57 2PX

U. K.

Phone: + 44(0)1248388296

Fax: + 44 (0) 12 48 37 10 53

Email: M.Whitham@bangor.ac.uk

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