NG2-expression as a marker for MRD: The molecule detected by moAb 7.1 can not be found on the surface of primitive CD34+ progenitor cells

J. Neudenberger; A. Rosemann; M. Hotfilder; C. Langebrake; D. Reinhardt; J. Vormoor

doi:10.1055/s-2004-828586

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Klin Padiatr 2004; 216 - 41
DOI: 10.1055/s-2004-828586

NG2-expression as a marker for MRD: The molecule detected by moAb 7.1 can not be found on the surface of primitive CD34+ progenitor cells

J Neudenberger ¹, A Rosemann ¹, M Hotfilder ¹, C Langebrake ¹, D Reinhardt ¹, J Vormoor ¹

¹Department of Pediatric Hematology and Oncology, University Children's Hospital Münster, Germany

Congress Abstract

The human homologue of the rat NG2 protein is being discussed as a potential marker for early minimal residual disease (MRD) detection in high risk 11q23-transformed leukemia. As recent studies have shown, the leukemic transformation can occure in CD34+ primitive stem/progenitor cells. Consequently a protein expressed on the leukemia initiating cell concurrent with the transformation, would be the key to early MRD detection. We analyzed bone marrow or peripheral blood samples of 8 patients positive for 11q23-aberrations by flow cytometry and determined the NG2 expression of CD117⁺, CD34⁺, CD19^-cells in ALL samples and of CD34⁺, CD38^-, CD33^- cells in AML samples. The results were identical for the whole sample collective: Both primitive progenitor cell populations did not express the NG2 molecule. Accordingly the molecule detected by moAb 7.1 is not suitable to detect the clonogenic leukemia initiating cell.