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Horm Metab Res 2006; 38(5): 308-313
DOI: 10.1055/s-2006-925404
Original Basic
© Georg Thieme Verlag KG Stuttgart · New York

Hyperthyroid Hearts Display a Phenotype of Cardioprotection Against Ischemic Stress: A Possible Involvement of Heat Shock Protein 70

C.  Pantos1 , V.  Malliopoulou1 , I.  Mourouzis1 , A.  Thempeyioti1 , I.  Paizis1 , A.  Dimopoulos1 , T.  Saranteas1 , C.  Xinaris1 , D.  V.  Cokkinos2
  • 1Department of Pharmacology, University of Athens
  • 21st Cardiology Department, Onassis Cardiac Surgery Center, Athens, Greece
Further Information

Publication History

Received 4 October 2005

Accepted after revision 21 December 2005

Publication Date:
23 May 2006 (online)

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Abstract

Hyperthyroid hearts are shown to display a phenotype of cardioprotection against ischemic stress, but the underlying signaling mechanisms remain largely unknown. The present study investigated the possible relation of HSP70 to the thyroid hormone induced cardioprotection. HSP70 is a redox-regulated molecular chaperone, and enhances cell survival under stress. Thyroxin (25 µg/100 g body weight) was administered to Wistar male rats for four days (THYR-4d) and two weeks (THYR-14d), respectively, while untreated animals served as controls (CON-4d, CON-14d). Isolated hearts from control and thyroxin treated rats were subjected to 20 min zero-flow ischemia followed by 45 min of reperfusion (I/R). The amount of HSP70 in the myocardium for THYR-14d was 1.85 times the levels of CON-14d (p < 0.05). The levels of HSP70 expression were no different between THYR-4d and CON-4d, p > 0.05. This was only accompanied by an increase in MDA levels (used as an index of oxidative stress) in THYR-14d compared to untreated hearts. These changes corresponded to a differential response of the heart to I/R; post-ischemic recovery of function was significantly increased in THYR-14d compared to CON-14d, and was no different between the THYR-4d and CON-4d hearts. In conclusion, long-term thyroxin administration results in increased tolerance of the myocardium to I/R and enhances the expression of HSP70 which may, at least in part, account for this response.

Key words

Thyroid hormone - heat shock proteins - heat stress - oxidative stress - ischemia reperfusion

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Constantinos Pantos

Department of Pharmacology

University of Athens · 75 Mikras Asias Ave. · 11527 Goudi · Athens · Greece

Fax: +30 (21) 07 70 51 85

Email: cpantos@cc.uoa.gr

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