Weight Loss with Long-term Intermittent Treatment with Oral Oleoyl-estrone in Lean Zucker Male Rats

Abstract

To determine whether or not the long-term intermittent treatment with oleoyl-estrone (OE) in rats induces a cumulative weight loss, adult male rats were treated with OE over three alternating 10-day periods, with a 30-day “recovery” period occurring between each period. At the end of the third treatment period, the rats were allowed to recover and were then mated with females. Each treatment period produced a decrease of ca. 7% in body weight with no rebound during the recovery periods, whereas weight changed at the same pace in controls. The greatest difference in body weight was observed during the last days of treatment. OE-treated rats retained their initial protein pools throughout the treatment. Furthermore, treated and control males remained fertile and were able to procreate. Thus, we can conclude that intermittent OE treatment induces a cumulative weight loss in adult male rats.

Key words

Oleoyl-estrone - intermittent treatment - fertility - weight loss - body protein

References

• 1 Esteve M, Savall P, Virgili J, Fernández-López JA, Remesar X, Alemany M. Modulation by leptin, insulin and corticosterone of oleoyl-estrone synthesis in cultured 3T3L1 cells.  Biosci Rep. 2001;  21 755-763
• 2 Fernández-Real JM, Sanchis D, Ricart W, Casamitjana R, Balada F, Remesar X, Alemany M. Plasma estrone-fatty acid ester levels are correlated with body fat mass in humans.  Clin Endocrinology. 1999;  50 253-260
• 3 Cabot C, Masanés R, Bulló M, García-Lorda P, Fernández-López JA, Salas-Salvadó J, Alemany M. Plasma acyl-estrone levels are altered in obese women.  Endocr Res. 2000;  26 465-476
• 4 Sanchis D, Balada F, Grasa MM, Virgili J, Peinado J, Monserrat C, Fernández-López JA, Remesar X, Alemany M. Oleoyl-estrone induces the loss of body fat in rats.  Int J Obes. 1996;  20 588-594
• 5 Grasa MM, Cabot C, Esteve M, Yubero P, Massanés RM, Blay MT, Vilà R, López-Martí J, Fernández-López JA, Remesar X, Alemany M. Daily oral oleoyl-estrone gavage induces a dose-dependent loss of fat in Wistar rats.  Obes Res. 2001;  9 202-209
• 6 Adán C, Cabot C, Vilà R, Grasa MM, Masanés RM, Esteve M, Estruch J, Fernández-López JA, Remesar X, Alemany M. Oleoyl-estrone treatment affects the ponderostat setting differently in lean and obese Zucker rats.  Int J Obes. 1999;  23 366-373
• 7 Alemany M, Fernández-López JA, Pietrobelli A, Granada M, Foz M, Remesar X. Pérdida de peso en un paciente con obesidad mórbida en tratamiento con oleoil-estrona.  Med Clin (Barc). 2003;  121 496-499
• 8 Cabot C, Grasa MM, Masanés RM, de Matteis R, Cinti S, Fernández-López JA, Remesar X, Alemany M. Oleoyl-estrone does not have direct estrogenic effects on rats.  Life Sci. 2001;  69 749-761
• 9 Masanés RM, Grasa MM, López-Martí J, Díaz-Silva M, Fernández-López JA, Remesar X, Alemany M. Zucker obese rats store less acyl-estrone than lean controls.  Int J Obes. 2003;  27 428-432
• 10 Mac Lusky NJ, Naftolin F. Sexual differentiation of the central nervous system.  Science. 1981;  211 1294-1303
• 11 Tena-Sempere M, Navarro J, Pinilla L, González LC, Huhtaniemi I, Aguilar E. Neonatal exposure to estrogen differentially alters estrogen receptor α and β mRNA expression in rat testis during postnatal development.  J Endocrinol. 2000;  165 345-357
• 12 López-Martí J, Díaz-Silva M, Salas A, Grasa MM, Fernández-López JA, Remesar X, Alemany M. Oleoyl-estrone induces the massive loss of body weight in Zucker fa/fa rats fed a high-energy diet.  J Nutr Biochem. 2000;  11 530-535
• 13 Folch J, Lees M, Sloane-Stanley GH. A simple method for the isolation and purification of total lipids from animal tissues.  J Biol Chem. 1957;  232 497-509
• 14 Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent.  J Biol Chem. 1951;  193 265-275
• 15 Salas A, Esteve M, Grasa MM, Remesar X. Rats treated with oleoyl-estrone maintain glucidic homeostasis: comparisons with a pair-fed model.  Br J Nutr. 2005;  94 738-745
• 16 Romero MM, Esteve M, Alemany M. Combined effects of oral oleoyl-estrone and limited food intake on body composition of young overweight male rats.  Int J Obes. 2006;  30 1149-1156

Correspondence