Pre- and Postnatal Protein Undernutrition Increases Hepatic Carnitine Palmitoyltransferase I Activity and Decreases Enzyme Sensitivity to Inhibitors in the Suckling Rat

M. Guzmán; I. R. Azzolin; C. C. Moulin; M. L. S. Perry

doi:10.1055/s-2007-1003365

Hormone and Metabolic Research, Table of Contents

Horm Metab Res 1992; 24(10): 471-473
DOI: 10.1055/s-2007-1003365

Originals Basic

Pre- and Postnatal Protein Undernutrition Increases Hepatic Carnitine Palmitoyltransferase I Activity and Decreases Enzyme Sensitivity to Inhibitors in the Suckling Rat

M. Guzmán¹ , I. R. Azzolin² , C. C. Moulin² , M. L. S. Perry²

¹Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Complutense University, Madrid, Spain
²Department of Biochemistry, Institute of Biosciences, UFRGS, Porto Alegre, RS, Brazil

Abstract

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Summary

Rats were pair-fed isocaloric diets containing either 25 % (control diet) or 6 % protein (low-protein diet) during the 5 weeks prior to conception and through the gestation and lactation periods; then, carnitine palmitoyltransferase I (CPT-I) activity was determined in liver and skeletal muscle mitochondria isolated from the corresponding pups. Maternal protein undernutrition increased the activity of hepatic CPT-I all along the suckling period, whereas the activity of the skeletal muscle enzyme was unaffected. Moreover, the sensitivity of hepatic CPT-I to inhibition by bothmalonyl-CoA and 4-hydroxyphenylglyoxylate was decreased in the low-protein group. These alterations in the properties of hepatic CPT-I may be involved in the appearance of hyperketonemia in the rat pup upon maternal administration of low-protein diets.

Key words

Protein Undernutrition - Carnitine Palmitoyltransferase I - Malonyl-CoA - 4-Hydroxyphenylglyoxylate - Rat-Pup Liver

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