Horm Metab Res 2008; 40(1): 24-28
DOI: 10.1055/s-2007-1004530
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

Effects of Continuous Low-carbohydrate Diet after Long-term Exercise on GLUT-4 Protein Content in Rat Skeletal Muscle

M. Kubota 1 , K. Koshinaka 2 , Y. Kawata 3 , T. Koike 3 , 4 , Y. Oshida 3 , 4
  • 1Department of Health Science, Faculty of Medicine, Kyoto University, Kyoto, Japan
  • 2Department of Health and Nutrition, Faculty of Medical Technology, Niigata University of Health and Welfare, Niigata, Japan
  • 3Department of Sports Medicine, Graduate School of Medicine, Nagoya University, Nagoya, Japan
  • 4Research Center of Health, Physical Fitness and Sports, Nagoya University, Nagoya, Japan
Further Information

Publication History

received 21.02.2007

accepted 05.06.2007

Publication Date:
19 December 2007 (eFirst)


Stimulation of AMPK and decreased glycogen levels in skeletal muscle have a deep involvement in enhanced insulin action and GLUT-4 protein content after exercise training. The present study examined the chronic effects of a continuous low-carbohydrate diet after long-term exercise on GLUT-4 protein content, glycogen content, AMPK, and insulin signaling in skeletal muscle. Rats were divided randomly into four groups: normal chow diet sedentary (N-Sed), low carbohydrate diet sedentary (L-Sed), normal chow diet exercise (N-Ex), and low carbohydrate diet exercise (L-Ex) groups. Rats in the exercise groups (N-Ex and L-Ex) were exercised by swimming for 6 hours/day in two 3-hour bouts separated by 45 minutes of rest. The 10-day exercise training resulted in a significant increase in the GLUT-4 protein content (p<0.01). Additionally, the GLUT-4 protein content in L-Ex rats was increased by 29% above that in N-Ex rats (p<0.01). Finally, the glycogen content in skeletal muscle of L-Ex rats was decreased compared with that of N-Ex rats. Taken together, we suggest that the maintenance of glycogen depletion after exercise by continuous low carbohydrate diet results in the increment of the GLUT-4 protein content in skeletal muscle.



Y. Oshida, MD, PhD 

Department of Sports Medicine

Graduate School of Medicine

Nagoya University

Nagoya 464-8601


Phone: +81/52/789 39 61

Fax: +81/52/789 39 57

Email: oshida@htc.nagoya-u.ac.jp