Horm Metab Res 2008; 40(1): 24-28
DOI: 10.1055/s-2007-1004530
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

Effects of Continuous Low-carbohydrate Diet after Long-term Exercise on GLUT-4 Protein Content in Rat Skeletal Muscle

M. Kubota 1 , K. Koshinaka 2 , Y. Kawata 3 , T. Koike 3 , 4 , Y. Oshida 3 , 4
  • 1Department of Health Science, Faculty of Medicine, Kyoto University, Kyoto, Japan
  • 2Department of Health and Nutrition, Faculty of Medical Technology, Niigata University of Health and Welfare, Niigata, Japan
  • 3Department of Sports Medicine, Graduate School of Medicine, Nagoya University, Nagoya, Japan
  • 4Research Center of Health, Physical Fitness and Sports, Nagoya University, Nagoya, Japan
Weitere Informationen


received 21.02.2007

accepted 05.06.2007

19. Dezember 2007 (online)


Stimulation of AMPK and decreased glycogen levels in skeletal muscle have a deep involvement in enhanced insulin action and GLUT-4 protein content after exercise training. The present study examined the chronic effects of a continuous low-carbohydrate diet after long-term exercise on GLUT-4 protein content, glycogen content, AMPK, and insulin signaling in skeletal muscle. Rats were divided randomly into four groups: normal chow diet sedentary (N-Sed), low carbohydrate diet sedentary (L-Sed), normal chow diet exercise (N-Ex), and low carbohydrate diet exercise (L-Ex) groups. Rats in the exercise groups (N-Ex and L-Ex) were exercised by swimming for 6 hours/day in two 3-hour bouts separated by 45 minutes of rest. The 10-day exercise training resulted in a significant increase in the GLUT-4 protein content (p<0.01). Additionally, the GLUT-4 protein content in L-Ex rats was increased by 29% above that in N-Ex rats (p<0.01). Finally, the glycogen content in skeletal muscle of L-Ex rats was decreased compared with that of N-Ex rats. Taken together, we suggest that the maintenance of glycogen depletion after exercise by continuous low carbohydrate diet results in the increment of the GLUT-4 protein content in skeletal muscle.



Y. Oshida, MD, PhD 

Department of Sports Medicine

Graduate School of Medicine

Nagoya University

Nagoya 464-8601


Telefon: +81/52/789 39 61

Fax: +81/52/789 39 57

eMail: oshida@htc.nagoya-u.ac.jp