Download PDF
Semin Liver Dis 1999; 19(1): 71-81
DOI: 10.1055/s-2007-1007099
ORIGINAL ARTICLE

© 1999 by Thieme Medical Publishers, Inc.

Gene Delivery to the Liver Using Simian Virus 40-derived Vectors

David S. Strayer, Mark A. Zern
  • Departments of Pathology and Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania
Further Information

Publication History

Publication Date:
17 March 2008 (online)

    Permissions and Reprints

ABSTRACT

We describe here the development and testing of simian virus 40 (SV40)-derived vectors to deliver foreign genetic material to the liver. Based on current understanding of the biology of wild-type SV40, it should be possible to exploit several important attributes of this virus, including efficient replication and gene expression, almost universal infec-tivity, and low immunogenicity if large T-antigen is deleted, to deliver DNA to the liver effectively. Our studies in cultured hepatocytes and in vivo, using both reporter constructs and transgenes of therapeutic interest, provide strong experimental support for this prediction. These successes indicate that SV40 may play an important role in gene delivery to the liver.

KEY WORDS

transduction - gene therapy - simian virus 40

      >