Insulin Action is Mimicked by Polyclonal Antireceptor Antibodies that Activate the Insulin Receptor Tyrosine Kinase

Summary

The insulin-like properties of anti-insulin receptor antibodies (P95 Ab) that have been characterized as being directed against the receptor β-subunit, were studied as probes to assess the interrelationship between insulin action and receptor phosphorylation.

When tested on intact cells, P95 Ab mimicked insulin effects. On isolated fat cells, they stimulated 2-deoxyglucose (2-DG) transport and lipogenesis and the P95 antibody maximal effects (173 and 232% of the control values, respectively) represented about 50% of the maximal effects elicited by insulin (317 and 475% of the control values). On cultured Zajdela hepatoma cells (ZHC cells), P95 Ab also mimicked insulin action on the incorporation of [U-¹⁴C]glucose into glycogen (158 and 207% of the control value for antibody- and insulin-treated cells, respectively). In all cases the antibody effects were dose-dependent, specific and, when maximal, were not additive with those elicited by insulin.

When tested in a cell-free system, P95 Ab faithfully reproduced insulin action on the phosphorylation of the receptor β-subunit. The maximal antibody and insulin effects (317 and 328% of the control value, respectively) were not additive. P95 Ab were also equally potent as insulin to stimulate the receptor-mediated phosphorylation of an exogenous substrate (365 and 379% of the control value in P95 antibody- and insulin-treated receptors, respectively). As well, P95 Ab proved as able as insulin in stimulating the tyrosine kinase activity of the receptor (89% of the hormone effect) when the activation was carried out in vivo.

Taken together, these results are consistent with a role for the kinase activity of the insulin receptor in mediating the action of insulin.

Key words

Insulin Receptor - Antireceptor Antibodies - Tyrosine Kinase - Glucose Metabolism