Effects of Opioid Peptides on Thyrotropin-Releasing Hormone Release from the Rat Caecum in Vitro

T. Mitsuma; Y. Hirooka; K. Nakata; T. Nogimori

doi:10.1055/s-2007-1009220

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Horm Metab Res 1989; 21(6): 301-304
DOI: 10.1055/s-2007-1009220

Originals Basic

Effects of Opioid Peptides on Thyrotropin-Releasing Hormone Release from the Rat Caecum in Vitro

T. Mitsuma¹ , Y. Hirooka¹ , K. Nakata¹ , T. Nogimori²

¹Fourth Department of Internal Medicine, Aichi Medical University, Nagakute, Aichi, Japan
²Department of Internal Medicine, Konanshowa Hospital, Konan, Aichi, Japan

Weitere Informationen

Publikationsverlauf

1988

1988

Publikationsdatum:
14. März 2008 (online)

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Summary

Effects of opioid peptides (β-endorphin, dynorphin (1-13). α-neoendorphin, β-neoendorphin, leucine-enkephalin, methionine-enkephalin) on the release of thyrotropin-releasing hormone (TRH) from the rat caecum were studied in vitro. The rat caecum was incubated in medium 199 with 1.0 mg/ml of bacitracin (pH 7.4) (medium). The amount of TRH release from the rat caecum into the medium was measured by radioimmunoassay.

The immunoreactive TRH (ir-TRH) release from the rat caecum was inhibited significantly in a dose-related manner with the addition of opioid peptides. The inhibitory effects of opioid peptides on ir-TRH release from the rat caecum were blocked with an addition of naloxone. The elution profile of acid-methanol-extracts of rat caecum on Sephadex G-10 was identical to that of synthetic TRH.

The findings suggest that opioid peptides inhibit TRH release from the rat caecum in vitro.

Key words

Thyrotropin-Releasing Hormone - Opioid Peptide - Caecum - Rat