Bioactivity of Human Growth Hormone in Urine from Acromegalic Patients

T. Tanaka; S. Umezawa; H. Yano; I. Hibi; Y. Shishiba; A. Teramoto; E. Ishikawa; Y. Murakami

doi:10.1055/s-2007-1009226

Hormone and Metabolic Research, Table of Contents

Horm Metab Res 1989; 21(6): 324-327
DOI: 10.1055/s-2007-1009226

Clinical

Bioactivity of Human Growth Hormone in Urine from Acromegalic Patients

T. Tanaka¹ , S. Umezawa¹ , H. Yano¹ , I. Hibi¹ , Y. Shishiba² , A. Teramoto³ , E. Ishikawa⁴ , Y. Murakami⁵

¹Endocrine Research Laboratory, National Children's Medical Research Center
²Department of Endocrinology and Metabolism, Toranomon Hospital
³Department of Neurosurgery, Police Hospital
⁴Department of Biochemistry, Miyazaki Medical College
⁵Sumitomo Pharmaceutical Co, Ltd., Tokyo, Japan

Abstract

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Summary

Bioactivity of hGH in urine from five acromegalic patients was determined by Nb2 bioassay and IM-9 receptor modulation assay (RMA). Urine samples were concentrated by immunoadsorbant chromatography, dialysis and lyophilization. The ratio of the bioactivity by Im-9 RMA and the immunoactivity by RIA was between 0.81 and 1.24(1.01 ± 0.19, mean ± SD). The ratio of the bioactivity by Nb2 bioassay and the immunoactivity ranged from 0.45 to 0.60 (1.37 ± 0.85).

Gel chromatography of hGH in urine samples measured by sensitive sandwich enzyme immunoassay showed that more than 97% of hGH activity was the 22K form.

Urinary hGH from acromegalic patients was bioactive in Nb2 bioassay and IM-9 RMA and the bioactivity showed a close correlation with the immunoactivity. The major immunoactive form of hGH (22K) seems to correspond to the bioactivity.

Key words

Bioactivity - Urinary Growth Hormone - Heterogeneity - Acromegaly

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