The hormonal regulation of thyroglobulin production has been studied using a clone
of the ovine thyroid cell line: OVNIS 6H. 3 among the 6 hormones proposed for serum
replacement are required for an optimal thyroglobulin production; insulin, hydrocortisone
and thyrotropin. Insulin alone stimulates thyroglobulin production. The presence of
insulin is also required to observe hydrocortisone and TSH stimulations. Newborn calf
serum inhibits thyroglobulin production. The best conditions for optimal thyroglobulin
expression and TSH responsiveness are obtained in serumfree medium supplemented with
5 μg/ml insulin, 100 nM hydrocortisone and 1 mU/ml TSH.
Thyroid
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Thyroglobulin
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Thyrotropin
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Insulin
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Hydrocortisone
