Horm Metab Res 1988; 20: 421-425
DOI: 10.1055/s-2007-1010851
© Georg Thieme Verlag, Stuttgart · New York

Isolation and Partial Characterization of Insulin of the Honeybee (Apis Mellifica)

V. Maier, P. Steiner, J. Fuchs, B. Pfeifle, M. Mezger, E. F. Pfeiffer
  • Medizinische Klinik und Poliklinik, Abteilung Innere Medizin I, Universität Ulm, Ulm, Germany
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Publication History

Publication Date:
14 March 2008 (online)


In the honeybee (Apis mellifica), insulin-like material was partially purified with acid ethanol extractions by a classic method for recovering insulin and following gel filtration on a Sephadex G-50 column. The preparations were characterized by their ability to cross-react with porcine insulin antibodies. Insulin-like biological activity was demonstrated using the insulin bioassay.

Stimulation of glucose oxidation or lipogenesis was measured by isolated rat adipocytes. Insulin seems to be more widespread in invertebrates than was previously assumed.