Summary
The present experiment was carried out to investigate the metabolism of palatinose
(6-0-α-D-glucopyranosyl-D-fructose) in the rat. The bolus injection of palatinose
(0.5 g/kg) in the tail vein of normal and Streptozotocin (STZ) diabetic rats caused
significant increments in glucose and insulin concentrations. However, in severe STZ
diabetic rats (> 300 mg/dl of fasting plasma glucose) no significant change in glucose
and insulin concentrations was observed. In liver perfusion, the gradual decrease
in glucose output from the normal and mild STZ diabetic rat livers perfused with 20
mM Krebs-Ringer-Tris buffer pH 7.4 was prevented by the addition of 5.5 mM palatinose
in the perfusate and fructose was detected in the effluent during the palatinose infusion.
The results indicate that palatinose is metabolized to glucose and fructose in both
normal and diabetic rat tissues, and this causes the increase in blood glucose concentration.
On the other hand, the direct stimulatory effect of insulin release from pancreatic
B-cell was not observed when the palatinose was infused into the isolated perfused
rat pancreas. The study suggest that palatinose administered parenterally is metabolized
by tissues and expected to be used as a source of fluid and energy supply.
Key-Words
Palatinose
-
Glood Glucose
-
Liver Perfusion
-
Pancreas Perfusion
-
Streptozotocin Diabetic Rat
