The Effect of Various Doses of Lazaroid U74389G on Lung Ischemia Reperfusion Injury

Abstract

Background: We investigated the effect of lazaroid U74389G, a potent inhibitor of lipid peroxidation, on ischemia-reperfusion injury at three different doses in the rat orthotopic left lung transplantation model. Methods: Four groups of reperfused lungs were studied. In group I (control) donor lungs were transplanted after 12 hours of preservation in University of Wisconsin (UW) Solution at 4°C. In groups II, III, and IV, lazaroid was used as an additive to UW Solution at concentrations of 30 μmol/L, 50 μoml/L, and 100 μmol/L respectively, and was also administered intravenously to the recipients 30 minutes before reperfusion after 12 hours of storage in the UW solution at 4 C. Results: After 1 hour of reperfusion, gas exchange function (p < 0.01), tissue lipid peroxide levels (p< 0.05) and histologic damage in reperfused lung allografts (p< 0.05) were significantly improved in lazaroidtreated group IV compared with control group I. Conclusions: These findings suggest that lazaroid U74389G ameliorates ischemia-reperfusion injury in rat lung transplants by inhibiting lipid peroxidation.

Key words

Lazaroid U74389G - Ischemia reperfusion injury - Lipid peroxidation - Lung transplantation