Summary
2-(3-Methyl-cinnamyl-hydrazono)-propionate (MCHP) is a new compound which effectively
lowers the blood glucose level in guinea pigs. 0.04 mmol/l MCHP inhibited glucose
formation from lactate, pyruvate and alanine, but not from dihydroxyacetone in the
perfused guinea pig liver. In the presence of both hexanoic acid and alanine, 0.04
mmol/l MCHP did not effect hepatic gluconeogenesis.
Gluconeogenesis was probably reduced by an inhibition of the pyruvate carboxylase:
1. The pattern of hepatic metabolite concentrations indicated a block between pyruvate
and the triose phosphates.
2. The intrahepatic concentration of acetyl-CoA was consistently decreased.
The decrease of the acetyl-CoA concentration could be explained by an influence of
MCHP on the fatty acid oxidations, which occurred at the carnitine palmitoyl transferase
(CPT) step. This hypothesis is supported by the fact that a medium chain fatty acid
not requiring CPT reversed the inhibition of gluconeogenesis by MCHP.
From all results reported it is concluded that transamination and oxidative phosphorylation
were not effected to a degree relevant for the inhibition of gluconeogenesis by 0.04
mmol/l MCHP.
Key-Words:
Methylcinnamylhydrazonopropionate
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Hepatic Gluconeogenesis
