Summary
The lipid-lowering effect of pantethine, a new drug affecting lipid metabolism, had
been evaluated in carbohydrate-induced hyperlipidemic rats.
Administration of the drug raised post-heparin lipolytic activities, the change being
due to an increase in lipoprotein lipase activity, whereas hepatic lipase activity
remained virtually unchanged.
Total lipoprotein lipase activity per g of adipose tissue increased in pantethine-treated
rats compared with controls. Furthermore, the soluble lipoprotein lipase of fat-pads
was fractionated by heparin-Sepharose affinity chromatography. The first active peak,
originated from the microsomal fractions, significantly increased after the drug treatment,
while the second one, originated from the plasma membranes, remained unchanged. The
increase in the microsomal lipoprotein lipase activity may be due to an increase in
intracellular synthesis of lipoprotein lipase enzyme proteins.
The heterogeneity of lipoprotein lipase of rat adipose tissues was ensured using affinity
chromatography on heparin-Sepharose
Key-Words:
Pantethine
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Hyperlipidemia
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Lipoprotein Lipase
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Hepatic Lipase
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Adipose Tissue
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Heterogeneity
