Effects of Adrenaline Pretreatment on In Vitro Binding of ¹²⁵I-Triiodothyronine to Nuclear Receptor, Intracellular Distribution of Endogenous Triiodothyronine and Activities of Alfa-Glycerophosphate Dehydrogenase and Malic Enzyme in Rat Liver

Summary

Especially coated adrenaline tablets (A) or placebo tablets (P) which release linearly the hormone were implanted in male Wistar rats. Six hours later animals were sacrificed and kinetic parameters of T3-¹²⁵I binding to nuclear receptor, intracellular distribution of endogenous T3 and activities of alfa-GPD and ME were investigated. The association constant values (Ka) of nuclear receptor were increased after pretreatment with 7.5, 15 and 45 mg A tablets and were 1.07, 1.35 and 1.48 × 10⁹M^-1 vs 0.85 × 10⁸M^-1 value seen after P. The maximal binding capacity (MBC) values decreased after pretreatment with the same doses of A and were 0.044, 0.036 and 0.025 pmol T3/100 μg DNA vs. 0.065 pmol T3/100 μg DNA in P pretreated. Adrenaline pretreatment significantly increased the amount of endogenous T3 present in liver nuclei while the amount of T3 present in cytosol decreased. Activity of mitochondrial alfa-GPD was increased after 15 and 45 mg of A. Significant rise of activity of cytosol ME was seen only after pretreatment with 45 mg of A.

Key-Words:

Adrenaline - Triiodothyronine - T3 Nuclear Receptor - T3 Intracellular Distribution - Activities of Alfa-GPD and ME