Summary
We determined erythrocyte concentration of phosphorylated glycolytic intermediates
and adenosine nucleotides in 9 non-ketotic diabetics under different conditions of
glycemic control. We showed a significant increase of G6P, F6P and FDP levels in the
9 diabetics with poor glycemic control, whereas other phosphorylated glycolytic intermediates
were in the normal range. After achieving fair glycemic control G6P, F6P and FDP fell
to normal value. G6P, F6P and FDP were well correlated with simultaneously determined
plasma glucose. Another 5 diabetics with poor glycemic control were connected to Biostator
in order to determine the time relationship between blood glucose and erythrocyte
levels of G6P, F6P and FDP. We demonstrated a prompt decrease to the normal values
of the phosphorylated glycolytic intermediates where blood glucose fell to normal
60-90 minutes after automatic insulin delivery. Finally, we determined RBC glycolytic
intermediates in 5 subjects with normal OGTT and in 5 subjects with normal fasting
plasma glucose but abnormal OGTT. In this group we observed a prompt and persistent
increase of G6P, F6P and FDP levels over the three hours hyperglycemia phase which
had been induced by glucose load. No significant increase was found in subjects with
normal OGTT.
Our results demonstrate that erythrocyte glycolytic metabolism is abnormal in diabetics,
depending strictly on the degree of glycemic control.
Key-Words:
Diabetes Mellitus
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RBC Metabolism
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Glycolytic Intermediates
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Biostator
