The Peroxisome Proliferator-activated Receptor Gamma Coactivator-1 Alpha Gene (PGC-1α) is Not Associated with Type 2 Diabetes Mellitus or Body Mass Index Among Hispanic and Non Hispanic Whites from Colorado

T. L. Nelson; T. E. Fingerlin; L. Moss; M. M. Barmada; R. E. Ferrell; J. M. Norris

doi:10.1055/s-2007-960495

Experimental and Clinical Endocrinology & Diabetes, Table of Contents

Exp Clin Endocrinol Diabetes 2007; 115(4): 268-275
DOI: 10.1055/s-2007-960495

Article

The Peroxisome Proliferator-activated Receptor Gamma Coactivator-1 Alpha Gene (PGC-1α) is Not Associated with Type 2 Diabetes Mellitus or Body Mass Index Among Hispanic and Non Hispanic Whites from Colorado

T. L. Nelson¹ ^, ² , T. E. Fingerlin² , L. Moss² , M. M. Barmada³ , R. E. Ferrell³ , J. M. Norris²

¹Department of Health and Exercise Science, Colorado State University, Fort Collins, CO
²Department of Preventive Medicine and Biometrics, University of Colorado at Denver and Health Sciences Center, Denver, CO
³Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA

Abstract

The objective of the study was to test for an association between type 2 diabetes mellitus (T2DM) and body mass index (BMI) and three single nucleotide polymorphisms (SNP)s in the peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) gene. We were also interested in whether these associations differed by tertiles of diet, physical activity or presence of polymorphisms in the peroxisome proliferator-activated receptor gamma (PPAR-γ) gene among Hispanics and Non-Hispanic Whites (NHW) from Colorado. We studied 216 Hispanic pedigrees (1850 nuclear families) and 236 NHW pedigrees (1240 families) from the San Luis Valley and Denver. We genotyped the Gly482Ser, Thr528Thr and Thr612Met polymorphisms in the PGC-1α gene and the Pro12Ala polymorphism of the PPAR-γ gene. Historical physical activity (average METS/week) as well as average dietary intake over the past year was assessed by self-report. Data were analyzed using the Family Based Association Test (FBAT) as well as generalized estimating equations (GEE). We did not find any significant association between three SNPs in the PGC-1α gene and T2DM in Hispanics or NHW; however, using FBAT, we found the common Thr612Thr allele of the PGC-1α gene to be associated with T2DM among Hispanic subjects carrying the rare Pro12Ala allele of the PPAR-γ gene (p=.003). We found similar associations when we considered a haplotype containing that allele (p=.002). However, the results of the GEE analysis did not confirm these findings: odds ratio (OR)=1.68, 95% CI (0.5, 5.2) suggesting these results may due to chance. BMI also did not show any consistent associations with the PGC-1α gene. In conclusion, we did not find an association between the PGC-1α gene and T2DM or BMI and there were no consistent interactions with diet, physical activity or the Pro12Ala polymorphism of the PPAR-γ gene.

Key words

type 2 diabetes - gene - environment interaction - Hispanic

Full Text

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Correspondence

T. L. Nelson

Colorado State University

Department of Health and Exercise Science

223 Moby

Fort Collins

CO 80523

Phone: 970/491 63 20

Fax: 970/491 04 45

Email: tnelson@cahs.colostate.edu