Subscribe to RSS
DOI: 10.1055/s-2007-965021
© Georg Thieme Verlag KG Stuttgart · New York
Effects of a Supplementation during Exercise and Recovery
Publication History
accepted after revision May 29, 2006
Publication Date:
11 May 2007 (online)

Abstract
The present study was designed to investigate whether a protein hydrolysate enriched in branched chain amino acids and antioxidants, trace and mineral elements, and vitamins would affect performance and fatigue. Eighteen sportsmen underwent testing before and after 28 days supplementation with either treatment in protein hydrolysate or placebo. Testing included exhaustive aerobic and anaerobic exercises with determination of blood lactate concentration through exercise and recovery and antioxidant status, but also measurements of maximal oxidative capacity (V·max) and citrate synthase activity (CS) from a resting muscle biopsy. Protein hydrolysate resulted in a significant decrease in fatigue indices, without affecting performances. A significant increase in enzymatic antioxidant and a decrease in oxidative damage were observed at rest after treatment but not with a placebo. Decrease in maximal blood lactate and improvement of blood lactate removal were only observed after protein hydrolysate treatment. Furthermore, CS increased significantly, whereas no change was observed in V·max. In conclusion, this protein hydrolysate treatment induced adaptations that may promote a decrease in fatigue during exercises, potentially explained by changes in parameters used to represent oxidative damage and antioxidant status at rest and changes in lactate metabolism.
Key words
supramaximal exercise - fatigue index - maximal blood lactate - oxidative damage
References
- 1 Alessio H M, Hagerman A E, Fulkerson B K, Ambrose J, Rice R E, Wiley R L. Generation of reactive oxygen species after exhaustive aerobic and isometric exercise. Med Sci Sports Exerc. 2000; 32 1576-1581
- 2 Balakrishnan S D, Anuradha C V. Exercise, depletion of antioxidants and antioxidant manipulation. Cell Biochem Funct. 1998; 16 269-275
- 3 Bergman B C, Horning M A, Casazza G A, Wolfel E E, Butterfield G E, Brooks G A. Endurance training increases gluconeogenesis during rest and exercise in men. Am J Physiol. 2000; 278 E244-E251
- 4 Brooks G A. Amino acid and protein metabolism during exercise and recovery. Med Sci Sports Exerc. 1987; 19 S150-S156
- 5 Brotherhood J R. Nutrition and sports performance. Sports Med. 1984; 1 350-389
- 6 Cachia O, Leger C L, Boulot P, Vernet M H, Michel F, Crastes de Paulet A, Descomps B. Red blood cell vitamin E concentrations in fetuses are related to but lower than those in mothers during gestation. A possible association with maternal lipoprotein (a) plasma levels. Am J Obstet Gynecol. 1995; 173 42-51
- 7 Clarkson P M, Thompson H S. Antioxidants: what role do they play in physical activity and health?. Am J Clin Nutr. 2000; 72 637S-646S
- 8 De Palo E F, Gatti R, Cappellin E, Schiraldi C, De Palo C B, Spinella P. Plasma lactate, GH and GH-binding protein levels in exercise following BCAA supplementation in athletes. Amino Acids. 2001; 20 1-11
- 9 Fitts R H. Muscle fatigue: the cellular aspects. Am J Sports Med. 1996; 24 S9-S13
- 10 Flohe L, Gunzler W A. Assays of glutathione peroxidase. Meth Enzymol. 1984; 105 114-121
- 11 Freund H, Gendry P. Lactate kinetics after short strenuous exercise in man. Eur J Appl Physiol. 1978; 39 123-135
- 12 Freund H, Lonsdorfer J, Oyono-Enguelle S, Lonsdorfer A, Bogui P. Lactate exchange and removal abilities in sickle cell patients and in untrained and trained healthy humans. J Appl Physiol. 1992; 73 2580-2587
- 13 Freund H R, Hanani M. The metabolic role of branched-chain amino acids. Nutrition. 2002; 18 287-288
- 14 Gutmann I, Wahlefeld A M. L-(+)-lactate determination with lactate dehydrogenase and NAD. Methods of Enzymatic Analysis. NY; Academic Press 1974: 1464-1472
- 15 Howarth K R, LeBlanc P J, Heigenhauser G J, Gibala M J. The effect of endurance training on muscle TCA cycle metabolism during exercise in humans. J Appl Physiol. 2004; 97 579-584
- 16 Ihara H, Shino Y, Morita Y, Kawaguchi E, Hashizume N, Yoshida M. Is skeletal muscle damaged by the oxidative stress following anaerobic exercise?. J Clin Lab Anal. 2001; 15 239-243
- 17 Lamb G D, Stephenson D G, Bangsbo J, Juel C. Point: counterpoint: lactic acid accumulation is an advantage/disadvantage during muscle activity. J Appl Physiol. 2006; 100 1410-1412
- 18 Letellier T, Malgat M, Coquet M, Moretto B, Parrot-Roulaud F, Mazat J P. Mitochondrial myopathy studies on permeabilized muscle fibers. Pediatr Res. 1992; 32 17-22
- 19 Lukaski H C, Johnson P E, Bolonchuch W W, Lykken L. Assessment of fat free mass using bioelectrical impedance measurements of the human body. Am J Clin Nutr. 1985; 41 810-817
- 20 MacLean D A, Graham T E, Saltin B. Stimulation of muscle ammonia production during exercise following branched-chain amino acid supplementation in humans. J Physiol. 1996; 493 909-922
- 21 Margaritis I, Palazzetti S, Rousseau A S, Richard M J, Favier A. Antioxidant supplementation and tapering exercise improve exercise-induced antioxidant response. J Am Coll Nutr. 2003; 22 147-156
- 22 Marzatico F, Pansarasa O, Bertorelli L, Somenzini L, Della Valle G. Blood free radical antioxidant enzymes and lipid peroxides following long-distance and lactacidemic performances in highly trained aerobic and sprint athletes. J Sports Med Phys Fitness. 1997; 37 235-239
- 23 Mettauer B, Zoll J, Sanchez H, Lampert E, Ribera F, Veksler V, Bigard X, Mateo P, Epailly E, Lonsdorfer J, Ventura-Clapier R. Oxidative capacity of skeletal muscle in heart failure patients versus sedentary or active control subjects. J Am Coll Cardiol. 2001; 38 947-954
- 24 Mourier A, Bigard A X, de Kerviler E, Roger B, Legrand H, Guezennec C Y. Combined effects of caloric restriction and branched-chain amino acid supplementation on body composition and exercise performance in elite wrestlers. Int J Sports Med. 1997; 18 47-55
- 25 Mourtzakis M, Graham T E. Glutamate ingestion and its effects at rest and during exercise in humans. J Appl Physiol. 2002; 93 1251-1259
- 26 Nemet D, Wolach B, Eliakim A. Proteins and amino acid supplementation in sports: are they truly necessary?. Isr Med Assoc J. 2005; 7 328-332
- 27 Preziosi P, Galan P, Herbeth B, Valeix P, Roussel A M, Malvy D, Paul-Dauphin A, Arnaud J, Richard M J, Briancon S, Favier A, Hercberg S. Effects of supplementation with a combination of antioxidant vitamins and trace elements, at nutritional doses, on biochemical indicators and markers of the antioxidant system in adult subjects. J Am Coll Nutr. 1998; 17 244-249
- 28 Ratel S, Duche P, Hennegrave A, Van Praagh E, Bedu M. Acid-base balance during repeated cycling sprints in boys and men. J Appl Physiol. 2002; 92 479-485
- 29 Robergs R A, Ghiasvand F, Parker D. Biochemistry of exercise-induced metabolic acidosis. Am J Physiol. 2004; 287 R502-R516
- 30 Saks V A, Veksler V I, Kuznetsov A V, Kay L, Sikk P, Tiivel T, Tranqui L, Olivares J, Winkler K, Wiedemann F, Kunz W S. Permeabilized cell and skinned fiber techniques in studies of mitochondrial function in vivo. Mol Cell Biochem. 1998; 184 81-100
- 31 Srere P. Citrate synthase. Meth Enzymol. 1969; 13 3-5
- 32 Striegel H, Simon P, Wurster C, Niess A M, Ulrich R. The use of nutritional supplements among master athletes. Int J Sports Med. 2006; 27 236-241
- 33 Sundgot-Borgen J, Berglund B, Torstveit M K. Nutritional supplements in Norwegian elite athletes - impact of international ranking and advisors. Scand J Med Sci Sports. 2003; 13 138-144
- 34 Thomas C, Perrey S, Lambert K, Hugon G, Mornet D, Mercier J. Monocarboxylate transporters, blood lactate removal after supramaximal exercise and fatigue indexes in humans. J Appl Physiol. 2005; 98 804-809
- 35 Thomas C, Sirvent P, Perrey S, Raynaud E, Mercier J. Relationships between maximal muscle oxidative capacity and blood lactate removal after supramaximal exercise and fatigue indexes in humans. J Appl Physiol. 2004; 97 2132-2138
- 36 van Hall G, MacLean D A, Saltin B, Wagenmakers A J. Mechanisms of activation of muscle branched-chain alpha-keto acid dehydrogenase during exercise in man. J Physiol. 1996; 494 899-905
- 37 Vukovich M D, Sharp R L, King D S, Kershishnik K. The effect of protein supplementation on lactate accumulation during submaximal and maximal exercise. Int J Sport Nutr. 1992; 2 307-316
- 38 Williamson J R, Cooper R H. Regulation of the citric acid cycle in mammalian systems. FEBS Lett. 1980; 117 (Suppl) K73-K85
- 39 Yagi K. A simple fluorometric assay for lipoperoxide in blood plasma. Biochem Med. 1976; 15 212-216
Dr. PhD Claire Thomas
Université Evry Val d'Essonne
UFR SFA, EA 3872, Departement STAPS
91000 Evry
France
Email: thomasclaire@wanadoo.fr