Thorac Cardiovasc Surg 2007; 55 - P_124
DOI: 10.1055/s-2007-967679

Activation of PARP enzyme and phosphorialion of protein kianse B (Akt) during and following cardiopulmonary bypass

B Gasz 1, S Bertok 2, B Rácz 2, B Sumegi 3, L Papp 4, E Röth 2, N Alotti 1
  • 1Zala Conty Hospital, Dep. of Cardiac Surgery and Department of Surgical Research and Techniques, University of Pécs, Medical Faculty, Zalaegerszeg, Hungary
  • 2Department of Surgical Research and Techniques, University of Pécs, Medical Faculty, Pécs, Hungary
  • 3Department of Biochemistry and Medical Chemistry, University of Pécs, Medical Faculty, Pécs, Hungary
  • 4Heart Institute, University of Pécs, Medical Faculty, Pécs, Hungary

Applying of cardiopulmonary bypass (CPB) is associated with several unwanted events, such as augmentation of inflammatory reactions, and increased production of oxygen free radicals (OFR). OFR induce DNA strand breaks triggering the activation of poly (ADP-ribose) polymerase-1 (PARP) enzime thus leading to NAD+ depletion resulting in cell death.

It is known that activation of protein kinase B (Akt) plays a pivotal role in the survival of cardiomyocytes during ischemia-reperfusion.

Purpose of this study was to determine the degree of oxidative stress and observe the activation of PARP and Akt during and after use of CPB in cardiac surgical-patients. Our data were compared with the values observed in patients undergoing coronary bypass grafting without CPB (OP)

30 patients were enrolled. (20 conventional coronary bypass operations with CPB and 10 OP operations were carried out.). During the operation and in the consecutive 7 days samples were taken from peripheral vein, to determine the oxidative stress parameters. PARP activation and Akt phosphorilation was assessed by flow cytometry.

More expressed PARP activation can be determined following CPB related to OPCAB patients. Akt activation decreased noticeably in course of CPB, following an early overactivation.

It can be concluded that CPB impairs Akt phosphorilation and increase PARP activation in patients. These outcomes about both PARP activation and Akt supression can suggest the practicability of PARP inhibition in patients receiving cardiac operation with CPB.

Supported by OTKA T038035