Int J Sports Med 1997; 18(2): 130-135
DOI: 10.1055/s-2007-972608

© Georg Thieme Verlag Stuttgart · New York

Acute Intensive Interval Training and In Vitro T-Lymphocyte Function

J. R. Hinton1 , D. G. Rowbottom2 , 3 , D. Keast2 , A. R. Morton3
  • 1Department of Biomedical Science, Anglia Polytechnic University, East Road, Cambridge, United Kingdom
  • 2Department of Microbiology, University of Western Australia, QE II Medical Centre, Nedlands, W.A., Australia
  • 3Department of Human Movement, University of Western Australia, Crawley, W.A., Australia
Further Information

Publication History

Publication Date:
09 March 2007 (online)

Five male endurance-trained runners completed an interval running session of 15 x 1-min intervals at 95 % VO2max. Venous blood samples were collected pre-exercise and then immediately, 30- and 60-minutes post-exercise. The response of cultures of total lymphocytes to mitogen (phytohaemagglutinin) were significantly reduced immediately after exercise, but returned to resting levels by 30-min of recovery. Conversely, the mitogen response of cultures of pure T-lymphocytes (CD4+ and CD8+ cells), separated using a magnetic separation technique, showed no significant change during the exercise and recovery periods. These data showed directly that there was no apparent change in the functional capability of T-lymphocytes following an intensive interval training session. Furthermore, there were significant changes in the composition of the total lymphocyte cultures immediately post-exercise; increased numbers of natural killer (NK) cells (CD56+) and T-suppressor cells (CD8+) and decreased numbers of T-helper cells (CD4+). There were also significant correlations between total mitogen response and the composition of the cultured lymphocytes. These data indicated that the large increases in NK cells, relative to T-cells, following intensive exercise, were the most likely cause of the reduced mitogen response of total lymphocyte cultures.