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Horm Metab Res 1999; 31(10): 580-582
DOI: 10.1055/s-2007-978799
Innovative Methods

© Georg Thieme Verlag Stuttgart · New York

A Simple Acute in Vivo Comparative Test for Sensitivity to Insulin in the Mouse

C. A. Williams1 , M.-F. Shih2 , P. V. Taberner1
  • 1Department of Pharmacology, School of Medical Sciences, University of Bristol, University Walk, Bristol, UK
  • 2Department of Pharmacy, Chai-Nan College of Pharmacy and Sciences, Tainan, Taiwan
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Publikationsverlauf

1999

1999

Publikationsdatum:
20. April 2007 (online)

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Abstract

A method is described for measuring the acute blood glucose response to an insulin challenge which requires only 6 samples of 20 µl of blood collected over a 4 hour period. This evaluation of sensitivity to insulin was validated by comparing the effects of gliclazide, metformin and a novel antidiabetic imidazoline compound (S22068) on the blood glucose response. The test distinguished between the insulin-secreting and hypoglycaemic action of gliclazide and the insulin-sensitizing actions of metformin and S22068. The test has the advantage that it can be repeated in the same animal after a period of recovery and thus enables the overall sensitivity to insulin to be compared before and after acute or chronic dose regimes.

Key words

Blood Glucose - Oral Hypoglycaemic Drugs

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